BMRB Entry 5661

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5661

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Rapid backbone 1H, 13C, and 15N assignment of the V1 domain of human PKC iota using the new program IBIS

Deposition date:

2003-01-15

Original release date:

2003-06-12

Authors:

Roehrl, Michael; Hyberts, Sven; Sun, Zhen-Yu; Fields, Alan; Wagner, Gerhard

Citation:

Citation: Roehrl, Michael; Hyberts, Sven; Sun, Zhen-Yu; Fields, Alan; Wagner, Gerhard. "Letter to the Editor: Rapid backbone 1H, 13C, and 15N assignment of the V1 domain of human PKC iota using the new program IBIS" J. Biomol. NMR 26, 373-374 (2003).

Assembly members:

Assembly members:
Protein Kinase C iota (V1 domain), polymer, 118 residues, 13223.89 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Protein Kinase C iota (V1 domain): GPLGSMSHTVAGGGSGDHSH QVRVKAYYRGDIMITHFEPS ISFEGLCNEVRDMCSFDNEQ LFTMKWIDEEGDPCTVSSQL ELEEAFRLYELNKDSELLIH VFPCVPERPGMPCPGEDK

Data sets:

assigned_chemical_shifts
- Shift_set_1

Data type	Count
13C chemical shifts	320
15N chemical shifts	102
1H chemical shifts	540

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PKC iota V1 monomer	1

Entities:

Entity 1, PKC iota V1 monomer 118 residues - 13223.89 Da.

1

GLY

PRO

LEU

GLY

SER

MET

SER

HIS

THR

VAL

2

ALA

GLY

SER

GLY

ASP

HIS

SER

HIS

3

GLN

VAL

ARG

VAL

LYS

ALA

TYR

ARG

GLY

4

ASP

ILE

MET

ILE

THR

HIS

PHE

GLU

PRO

SER

5

ILE

SER

PHE

GLU

GLY

LEU

CYS

ASN

GLU

VAL

6

ARG

ASP

MET

CYS

SER

PHE

ASP

ASN

GLU

GLN

7

LEU

PHE

THR

MET

LYS

TRP

ILE

ASP

GLU

8

GLY

ASP

PRO

CYS

THR

VAL

SER

GLN

LEU

9

GLU

LEU

GLU

ALA

PHE

ARG

LEU

TYR

GLU

10

LEU

ASN

LYS

ASP

SER

GLU

LEU

ILE

HIS

11

VAL

PHE

PRO

CYS

VAL

PRO

GLU

ARG

PRO

GLY

12

MET

PRO

CYS

PRO

GLY

GLU

ASP

LYS

Samples:

PKC_iota_V1_sample_1: Protein Kinase C iota (V1 domain), [U-13C; U-15N], 1.0 mM; Tris-HCl 20 mM; NaCl 150 mM; CaCl2 1 mM; DTT 1 mM

PKC_iota_V1_sample_2: Protein Kinase C iota (V1 domain), [U-15N], 1.0 mM; Tris-HCl 20 mM; NaCl 150 mM; CaCl2 1 mM; DTT 1 mM

Condition_1: ionic strength: 0.15 M; pH: 7.4; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	Condition_1
HNCA	not available	not available	Condition_1
HN(CO)CA	not available	not available	Condition_1
HN(CA)CB	not available	not available	Condition_1
HN(COCA)CB	not available	not available	Condition_1
HNCO	not available	not available	Condition_1
HN(CA)CO	not available	not available	Condition_1
(H)CC(CO)NH-TOCSY	not available	not available	Condition_1

Software:

PROSA v3.7 - data processing

XEASY v1.3.13 - data visualization

IBIS - semi-automated iterative assignment

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz

DBJ	BAG37741 BAG70257 BAJ20829
EMBL	CAH93307
GB	AAA60171 AAB17011 AAH22016 AAQ02606 AIC62578
REF	NP_001126946 NP_002731 XP_001516677 XP_002716448 XP_002759436
SP	P41743 Q5R4K9
AlphaFold	P41743 Q5R4K9