BMRB Entry 5659

Title:
1H, 13C and 15N Backbone Assignments for the Human Rhinovirus-14 3C Protease
Deposition date:
2003-01-13
Original release date:
2006-07-21
Authors:
Bjorndahl, Trent; Monzavi, Hassan; Wishart, David
Citation:

Citation: Bjorndahl, Trent; Monzavi, Hassan; Wishart, David. "Letter to the Editor: Backbone 1H, 15N and 13C assignments for the human rhinovirus 3C protease (serotype 14) "  J. Biomol. NMR 26, 85-86 (2003).

Assembly members:

Assembly members:
HRV14-3C, polymer, 182 residues, 19996.90 Da.

Natural source:

Natural source:   Common Name: Human Rhinovirus-14   Taxonomy ID: 12131   Superkingdom: Virus   Kingdom: Picornaviridae   Genus/species: Rhinovirus not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3a

Data sets:
Data typeCount
1H chemical shifts1163
13C chemical shifts683
15N chemical shifts159

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human Rhinovirus-14 3C Protease1

Entities:

Entity 1, Human Rhinovirus-14 3C Protease 182 residues - 19996.90 Da.

1   GLYPROASNTHRGLUPHEALALEUSERLEU
2   LEUARGLYSASNILEMETTHRILETHRTHR
3   SERLYSGLYGLUPHETHRGLYLEUGLYILE
4   HISASPARGVALCYSVALILEPROTHRHIS
5   ALAGLNPROGLYASPASPVALLEUVALASN
6   GLYGLNLYSILEARGVALLYSASPLYSTYR
7   LYSLEUVALASPPROGLUASNILEASNLEU
8   GLULEUTHRVALLEUTHRLEUASPARGASN
9   GLULYSPHEARGASPILEARGGLYPHEILE
10   SERGLUASPLEUGLUGLYVALASPALATHR
11   LEUVALVALHISSERASNASNPHETHRASN
12   THRILELEUGLUVALGLYPROVALTHRMET
13   ALAGLYLEUILEASNLEUSERSERTHRPRO
14   THRASNARGMETILEARGTYRASPTYRALA
15   THRLYSTHRGLYGLNCYSGLYGLYVALLEU
16   CYSALATHRGLYLYSILEPHEGLYILEHIS
17   VALGLYGLYASNGLYARGGLNGLYPHESER
18   ALAGLNLEULYSLYSGLNTYRPHEVALGLU
19   LYSGLN

Samples:

sample_1: HRV14-3C, [U-15N], 1 mM; KH2PO4 20 mM; NaCl 0 mM

sample_2: HRV14-3C, [U-15N; U-13C], 1 mM; KH2PO4 20 mM; NaCl 0 mM

conditions_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCACONNHnot availablenot availablenot available
HNHAnot availablenot availablenot available
15N-HSQCnot availablenot availablenot available

Software:

NMRPIPE - specta processing

NMRVIEW - peak picking, spectral analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

PDB
EMBL CAA25565
GB AAA45756 AAA45757 AAA45758
REF NP_041009 NP_740524
SP P03303
AlphaFold P03303

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks