BMRB Entry 5626

Title:
1H 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein
Deposition date:
2002-12-18
Original release date:
2003-06-10
Authors:
Shimizu, Masato
Citation:

Citation: Shimizu, Masato; Hiroaki, Hidekazu; Kohda, Daisuke; Morita, Eugene; Hotta, Shigeki; Morikawa, Kosuke. "Letter to the editor: 1H 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein"  J. Biomol. NMR 26, 277-278 (2003).

Assembly members:

Assembly members:
glial cell missing, polymer, 159 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
13C chemical shifts318
15N chemical shifts145
1H chemical shifts145

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GCM monomer1

Entities:

Entity 1, GCM monomer 159 residues - Formula weight is not available

1   GLYPROALATHRLYSSERARGVALALAILE
2   ASPTRPASPILEASNASPSERLYSMETPRO
3   SERVALGLYGLUPHEASPASPPHEASNASP
4   TRPSERASNGLYHISCYSARGLEUILETYR
5   SERVALGLNSERASPGLUALAARGLYSHIS
6   ALASERGLYTRPALAMETARGASNTHRASN
7   ASNHISASNVALASNILELEULYSLYSSER
8   CYSLEUGLYVALLEULEUCYSSERALALYS
9   CYSLYSLEUPROASNGLYALASERVALHIS
10   LEUARGPROALAILECYSASPLYSALAARG
11   ARGLYSGLNGLNGLYLYSGLNCYSPROASN
12   ARGASNCYSASNGLYARGLEUGLUILEGLN
13   ALACYSARGGLYHISCYSGLYTYRPROVAL
14   THRHISPHETRPARGARGASPGLYASNGLY
15   ILETYRPHEGLNALALYSGLYTHRHISASP
16   HISPROARGPROGLUALALYSGLYSER

Samples:

sample_1: glial cell missing, [U-99% 13C; U-99% 15N], 0.6 mM; potasium phosptae buffer 20 mM; KCl 0.3 M; Glycerol 10%

condition_number_1: ionic strength: 0.66 M; pH*: 6.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYnot availablenot availablenot available
1H-15N TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

NMRPipe vrelease 2002 -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

DBJ BAA10905
GB AAC46912 AAC47808 AAF52790 ABK30923 AGB92827
REF NP_001260292 NP_477108 XP_001969320 XP_002036350 XP_002088946
SP Q27403
AlphaFold Q27403

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks