BMRB Entry 5616
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5616
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Wiesner, Silke; Stier, Gunter; Sattler, Michael; Macias, Maria. "Solution Structure and Ligand Recognition of the WW Domain Pair of the Yeast
Splicing Factor Prp40" J. Mol. Biol. 324, 807-822 (2002).
PubMed: 12460579
Assembly members:
WW domain pair of pre-mRNA processing protein 40, polymer, 86 residues, 10251 Da.
Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9d
Entity Sequences (FASTA):
WW domain pair of pre-mRNA processing protein 40: MKHHHHHHPMGMSIWKEAKD
ASGRIYYYNTLTKKSTWEKP
KELISQEELLLRENGWKAAK
TADGKVYYYNPTTRETSWTI
PAFEKK
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 517 |
| 13C chemical shifts | 280 |
| 15N chemical shifts | 85 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Prp40, WW1-2 | 1 |
Entities:
Entity 1, Prp40, WW1-2 86 residues - 10251 Da.
| 1 | MET | LYS | HIS | HIS | HIS | HIS | HIS | HIS | PRO | MET | ||||
| 2 | GLY | MET | SER | ILE | TRP | LYS | GLU | ALA | LYS | ASP | ||||
| 3 | ALA | SER | GLY | ARG | ILE | TYR | TYR | TYR | ASN | THR | ||||
| 4 | LEU | THR | LYS | LYS | SER | THR | TRP | GLU | LYS | PRO | ||||
| 5 | LYS | GLU | LEU | ILE | SER | GLN | GLU | GLU | LEU | LEU | ||||
| 6 | LEU | ARG | GLU | ASN | GLY | TRP | LYS | ALA | ALA | LYS | ||||
| 7 | THR | ALA | ASP | GLY | LYS | VAL | TYR | TYR | TYR | ASN | ||||
| 8 | PRO | THR | THR | ARG | GLU | THR | SER | TRP | THR | ILE | ||||
| 9 | PRO | ALA | PHE | GLU | LYS | LYS |
Samples:
15N-labeled_sample: WW domain pair of pre-mRNA processing protein 40, [U-100% 15N], 1.5 mM
15N_13C_labeled_sample: WW domain pair of pre-mRNA processing protein 40, [U-100% 13C; U-100% 15N], 1.5 mM
Exp_Condition: pH: 6.3; temperature: 295 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| HN-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| CBCANH | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| HNHA-J | not available | not available | not available |
| HC-HSQC | not available | not available | not available |
| HC(CO)NH | not available | not available | not available |
| C(CO)NH | not available | not available | not available |
| HC(C)H-TOCSY | not available | not available | not available |
| 13C-NOESY | not available | not available | not available |
| 15N-NOESY | not available | not available | not available |
Software:
XEASY -
NMR spectrometers:
- Bruker DRX 500 MHz
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA24712 |
| EMBL | CAA81847 CAY81071 |
| GB | AAB24902 AHY76231 EDN59896 EDV13075 EDZ70922 |
| REF | NP_012913 |
| SP | P33203 |
| TPG | DAA09144 |
| AlphaFold | P33203 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks