BMRB Entry 5612

Title:
Structure of the Plant Defensin PhD1 from Petunia Hybrida   PubMed: 12846570
Deposition date:
2002-12-02
Original release date:
2003-08-08
Authors:
Janssen, B.; Schirra, H.; Lay, F.; Anderson, M.; Craik, D.
Citation:

Citation: Janssen, B.; Schirra, H.; Lay, F.; Anderson, M.; Craik, D.. "Structure of Petunia hybrida Defensin 1, a Novel Plant Defensin with Five Disulfide Bonds"  Biochemistry 42, 8214-8222 (2003).

Assembly members:

Assembly members:
PhD1, polymer, 47 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: garden petunia   Taxonomy ID: 4102   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Petunia x hybrida

Experimental source:   Production method: .

Experimental source:

Natural source:   Common Name: garden petunia   Taxonomy ID: 4102   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Petunia x hybrida

Experimental source:   Production method: .

Entity Sequences (FASTA):

Data sets:
Data typeCount
coupling constants13

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1floral defensin-like protein 11

Entities:

Entity 1, floral defensin-like protein 1 47 residues - Formula weight is not available

1   ALATHRCYSLYSALAGLUCYSPROTHRTRP
2   ASPSERVALCYSILEASNLYSLYSPROCYS
3   VALALACYSCYSLYSLYSALALYSPHESER
4   ASPGLYHISCYSSERLYSILELEUARGARG
5   CYSLEUCYSTHRLYSGLUCYS

Samples:

sample_1: PhD1 0.94 mM; H2O 90%; D2O 10%

sample_2: PhD1 0.94 mM; H2O 99%; D2O 1%

sample_cond_1: ionic strength: 0.94 mM; pH: 3.1; pressure: 1 atm; temperature: 310 K

sample_cond_2: ionic strength: 0.94 mM; pH: 3.1; pressure: 1 atm; temperature: 280 K

Experiments:

NameSampleSample stateSample conditions
E-COSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

XWINNMR v2.6 - collection, processing

CCNMR vglxccExplorer - data analysis

X-PLOR v3.851 - structure solution

CNS v1.1 - refinement, structure solution

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

PDB
GenBank AAN64750
SWISS-PROT Q8H6Q1