BMRB Entry 5603

Name: Chemical shift assignment for human proguanylin
Published: 2003-08-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5603

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical shift assignment for human proguanylin

Deposition date:

2002-11-28

Original release date:

2003-08-01

Authors:

Lauber, Thomas; Neudecker, Philipp; Roesch, Paul; Marx, Ute

Citation:

Citation: Lauber, Thomas; Neudecker, Philipp; Rosch, Paul; Marx, Ute. "Solution Structure of Human Proguanylin: The Role of a Hormone Prosequence" J. Biol. Chem. 278, 24118-24124 (2003).
PubMed: 12707255

Assembly members:

Assembly members:
proguanylin, polymer, 96 residues, 10491 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
proguanylin: GPVTVQDGNFSFSLESVKKL KDLQEPQEPRVGKLRNFAPI PGEPVVPILCSNPNFPEELK PLCKEPNAQEILQRLEEIAE DPGTCEICAYAACTGC

Data sets:

assigned_chemical_shifts
- shift_set_1
coupling_constants
- J_values_set_1

Data type	Count
1H chemical shifts	635
13C chemical shifts	268
15N chemical shifts	95
coupling constants	30

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	proguanylin	1

Entities:

Entity 1, proguanylin 96 residues - 10491 Da.

1

GLY

PRO

VAL

THR

VAL

GLN

ASP

GLY

ASN

PHE

2

SER

PHE

SER

LEU

GLU

SER

VAL

LYS

LEU

3

LYS

ASP

LEU

GLN

GLU

PRO

GLN

GLU

PRO

ARG

4

VAL

GLY

LYS

LEU

ARG

ASN

PHE

ALA

PRO

ILE

5

PRO

GLY

GLU

PRO

VAL

PRO

ILE

LEU

CYS

6

SER

ASN

PRO

ASN

PHE

PRO

GLU

LEU

LYS

7

PRO

LEU

CYS

LYS

GLU

PRO

ASN

ALA

GLN

GLU

8

ILE

LEU

GLN

ARG

LEU

GLU

ILE

ALA

GLU

9

ASP

PRO

GLY

THR

CYS

GLU

ILE

CYS

ALA

TYR

10

ALA

CYS

THR

GLY

CYS

Samples:

sample_1: proguanylin, [U-15N; U-13C], 0.8 – 1.1 mM; phosphate buffer 100 mM

ex-cond_1: pH: 6.0; temperature: 293 K; ionic strength: 0.1 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	ex-cond_1
1H-15N NOESY-HSQC	sample_1	not available	ex-cond_1
1H-15N TOCSY-HSQC	sample_1	not available	ex-cond_1
HNHA	sample_1	not available	ex-cond_1
15N HMQC-NOESY-HSQC	sample_1	not available	ex-cond_1
HNCO	sample_1	not available	ex-cond_1
HNCA	sample_1	not available	ex-cond_1
HNCACB	sample_1	not available	ex-cond_1
CBCA(CO)NH	sample_1	not available	ex-cond_1
HBHA(CBCACO)NH	sample_1	not available	ex-cond_1
H(CCO)NH	sample_1	not available	ex-cond_1
C(CO)NH	sample_1	not available	ex-cond_1
13C-CTHSQC	sample_1	not available	ex-cond_1
13C-NOESY-HSQC	sample_1	not available	ex-cond_1

Software:

NMRview v5.0.4 -

NMR spectrometers:

Bruker DRX 600 MHz
Bruker AVANCE 800 MHz

PDB	1O8R
EMBL	CAC22258
GB	AAA35915 AAA58625 AAI40429 AAI56567 EAX07168
REF	NP_291031 XP_003812931
SP	Q02747
AlphaFold	Q02747