BMRB Entry 5595

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5595

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Letter to the Editor: 1H, 13C, 15N backbone resonance assignments of apo-NosL, a novel 18.5 kDa copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes

Deposition date:

2002-11-21

Original release date:

2004-03-15

Authors:

Taubner, Lara; McGuirl, Michele; Dooley, David; Copie, Valerie

Citation:

Citation: Taubner, Lara; McGuirl, Michele; Dooley, David; Copie, Valerie. "Letter to the Editor: 1H, 13C, 15N backbone and sidechain resonance assignments of apo-NosL, a novel copper(I) binding protein from the nitrous oxide reductase gene cluster of Achromobacter cycloclastes" J. Biomol. NMR 29, 211-212 (2004).
PubMed: 15014237

Assembly members:

Assembly members:
NosL, polymer, 175 residues, 18538 Da.

Natural source:

Natural source: Common Name: Denitrifying bacteria Taxonomy ID: 223 Superkingdom: Eubacteria Kingdom: not available Genus/species: Achromobacter cycloclastes

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-20b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NosL: MDKEDVAQSIVPQDMTPETL GHYCQMNLLEHPGPKAQIFL EGSPAPLFFSQVRDAIAYAR GPEQIAPILVIYVNDMGAAG ATWDQPGDGNWIAADKAFYV VGSARRGGMGAPEAVPFSSR DEAAAFVLAEGGQVLALADI TDAMVLTPVETGSEPRADDE DYLGRLRALPHPAGG

Data sets:

assigned_chemical_shifts
- 15N-13C_NosL_shifts

Data type	Count
1H chemical shifts	846
13C chemical shifts	596
15N chemical shifts	148

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NosL monomer	1

Entities:

Entity 1, NosL monomer 175 residues - 18538 Da.

1

MET

ASP

LYS

GLU

ASP

VAL

ALA

GLN

SER

ILE

2

VAL

PRO

GLN

ASP

MET

THR

PRO

GLU

THR

LEU

3

GLY

HIS

TYR

CYS

GLN

MET

ASN

LEU

GLU

4

HIS

PRO

GLY

PRO

LYS

ALA

GLN

ILE

PHE

LEU

5

GLU

GLY

SER

PRO

ALA

PRO

LEU

PHE

SER

6

GLN

VAL

ARG

ASP

ALA

ILE

ALA

TYR

ALA

ARG

7

GLY

PRO

GLU

GLN

ILE

ALA

PRO

ILE

LEU

VAL

8

ILE

TYR

VAL

ASN

ASP

MET

GLY

ALA

GLY

9

ALA

THR

TRP

ASP

GLN

PRO

GLY

ASP

GLY

ASN

10

TRP

ILE

ALA

ASP

LYS

ALA

PHE

TYR

VAL

11

VAL

GLY

SER

ALA

ARG

GLY

MET

GLY

12

ALA

PRO

GLU

ALA

VAL

PRO

PHE

SER

ARG

13

ASP

GLU

ALA

PHE

VAL

LEU

ALA

GLU

14

GLY

GLN

VAL

LEU

ALA

LEU

ALA

ASP

ILE

15

THR

ASP

ALA

MET

VAL

LEU

THR

PRO

VAL

GLU

16

THR

GLY

SER

GLU

PRO

ARG

ALA

ASP

GLU

17

ASP

TYR

LEU

GLY

ARG

LEU

ARG

ALA

LEU

PRO

18

HIS

PRO

ALA

GLY

Samples:

15N_13C_sample: NosL, [U-15N; U-13C], 1.0 mM; DTT, [U-2D], 1.0 mM; EDTA 1.0 mM; D2O 10%; sodium phosphate 100 mM

15N_13C_NosL_cond: pH*: 6.5; temperature: 303 K; ionic strength: 0.1 M

Experiments:

Name	Sample	Sample state	Sample conditions
15N HSQC	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
CCONH	not available	not available	not available
HCCONH	not available	not available	not available
HBHACONH	not available	not available	not available
HNCA	not available	not available	not available
HNCO	not available	not available	not available
15N-TOCSY	not available	not available	not available
15N-NOESY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available

Software:

NMRPipe - processing, peak picking, peak tables

PIPP - processing, peak picking, peak tables

XWINNMR v3.1 - processing

NMR spectrometers:

BRUKER DRX 600 MHz

PDB	2HPU 2HQ3
EMBL	CAA75429
GB	AAD09161 KDP72987
REF	WP_029742711 WP_034799000