BMRB Entry 5592

Name: Backbone resonance assignments for the human GMEB-1 SAND domain
Published: 2003-03-19

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5592

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignments for the human GMEB-1 SAND domain

Deposition date:

2002-11-18

Original release date:

2003-03-19

Authors:

Bottomley, Matthew; Sattler, Michael

Citation:

Citation: Bottomley, Matthew; Sattler, Michael. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the SAND domains from glucocorticoid modulatory element binding proteins-1 and -2" J. Biomol. NMR 25, 259-260 (2003).

Assembly members:

Assembly members:
SAND domain from human GMEB1 protein, polymer, 97 residues, 11022 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Modified pET-24d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAND domain from human GMEB1 protein: GAMEDMEIAYPITCGESKAI LLWKKFVCPGINVKCVKFND QLISPKHFVHLAGKSTLKDW KRAIRLGGIMLRKMMDSGQI DFYQHDKVCSNTCRSTK

Data sets:

assigned_chemical_shifts
- CSR_1
coupling_constants
- J_values_set_1

Data type	Count
1H chemical shifts	192
13C chemical shifts	270
15N chemical shifts	92
coupling constants	33

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Human GMEB1 extended SAND domain	1
2	ZINC (II) ION	2

Entities:

Entity 1, Human GMEB1 extended SAND domain 97 residues - 11022 Da.

1

GLY

ALA

MET

GLU

ASP

MET

GLU

ILE

ALA

TYR

2

PRO

ILE

THR

CYS

GLY

GLU

SER

LYS

ALA

ILE

3

LEU

TRP

LYS

PHE

VAL

CYS

PRO

GLY

4

ILE

ASN

VAL

LYS

CYS

VAL

LYS

PHE

ASN

ASP

5

GLN

LEU

ILE

SER

PRO

LYS

HIS

PHE

VAL

HIS

6

LEU

ALA

GLY

LYS

SER

THR

LEU

LYS

ASP

TRP

7

LYS

ARG

ALA

ILE

ARG

LEU

GLY

ILE

MET

8

LEU

ARG

LYS

MET

ASP

SER

GLY

GLN

ILE

9

ASP

PHE

TYR

GLN

HIS

ASP

LYS

VAL

CYS

SER

10

ASN

THR

CYS

ARG

SER

THR

LYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1

ZN

Samples:

Sample_1: SAND domain from human GMEB1 protein, [U-98% 15N], 1.0 mM; sodium phosphate 20 mM; NaCl 0.2 M; DTT 3 mM; H2O 90%; D2O 10%

Sample_2: SAND domain from human GMEB1 protein, [U-98% 13C; U-98% 15N], 1.0 mM; sodium phosphate 20 mM; NaCl 0.2 M; DTT 3 mM; H2O 90%; D2O 10%

condition_1: pH: 6.0; temperature: 300 K; ionic strength: 0.22 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	condition_1
3D HNCO	not available	not available	condition_1
3D HNCA	not available	not available	condition_1
3D HNHA	not available	not available	condition_1
3D CBCANH	not available	not available	condition_1
3D CBCA(CO)NH	not available	not available	condition_1
3D 1H-1H-15N NOESY	not available	not available	condition_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz

SWISS-PROT	Q9JL60 Q9Y692 Q2HJ87
REF	NP_077808 NP_006573 NP_064669 NP_001039940 NP_001116464
GenBank	AAG01189 AAH01473 AAD51352 AAF72874 AAD39355
EMBL	CAM14478 CAH72427 CAH72428 CAH72426
DBJ	BAG37824 BAE22107 BAG37635 BAA91410
PDB	1OQJ