BMRB Entry 5592

Title:
Backbone resonance assignments for the human GMEB-1 SAND domain
Deposition date:
2002-11-18
Original release date:
2003-03-19
Authors:
Bottomley, Matthew; Sattler, Michael
Citation:

Citation: Bottomley, Matthew; Sattler, Michael. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the SAND domains from glucocorticoid modulatory element binding proteins-1 and -2"  J. Biomol. NMR 25, 259-260 (2003).

Assembly members:

Assembly members:
SAND domain from human GMEB1 protein, polymer, 97 residues, 11022 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: Modified pET-24d

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts192
13C chemical shifts270
15N chemical shifts92
coupling constants33

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human GMEB1 extended SAND domain1
2ZINC (II) ION2

Entities:

Entity 1, Human GMEB1 extended SAND domain 97 residues - 11022 Da.

1   GLYALAMETGLUASPMETGLUILEALATYR
2   PROILETHRCYSGLYGLUSERLYSALAILE
3   LEULEUTRPLYSLYSPHEVALCYSPROGLY
4   ILEASNVALLYSCYSVALLYSPHEASNASP
5   GLNLEUILESERPROLYSHISPHEVALHIS
6   LEUALAGLYLYSSERTHRLEULYSASPTRP
7   LYSARGALAILEARGLEUGLYGLYILEMET
8   LEUARGLYSMETMETASPSERGLYGLNILE
9   ASPPHETYRGLNHISASPLYSVALCYSSER
10   ASNTHRCYSARGSERTHRLYS

Entity 2, ZINC (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

Sample_1: SAND domain from human GMEB1 protein, [U-98% 15N], 1.0 mM; sodium phosphate 20 mM; NaCl 0.2 M; DTT 3 mM; H2O 90%; D2O 10%

Sample_2: SAND domain from human GMEB1 protein, [U-98% 13C; U-98% 15N], 1.0 mM; sodium phosphate 20 mM; NaCl 0.2 M; DTT 3 mM; H2O 90%; D2O 10%

condition_1: pH: 6.0; temperature: 300 K; ionic strength: 0.22 M

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablecondition_1
3D HNCOnot availablenot availablecondition_1
3D HNCAnot availablenot availablecondition_1
3D HNHAnot availablenot availablecondition_1
3D CBCANHnot availablenot availablecondition_1
3D CBCA(CO)NHnot availablenot availablecondition_1
3D 1H-1H-15N NOESYnot availablenot availablecondition_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 600 MHz

Related Database Links:

SWISS-PROT Q9JL60 Q9Y692 Q2HJ87
REF NP_077808 NP_006573 NP_064669 NP_001039940 NP_001116464
GenBank AAG01189 AAH01473 AAD51352 AAF72874 AAD39355
EMBL CAM14478 CAH72427 CAH72428 CAH72426
DBJ BAG37824 BAE22107 BAG37635 BAA91410
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks