BMRB Entry 5584

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5584

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

13C Chemical Shift Assignments of Cu(II) plastocyanin from Synechocystis sp. PCC6803

Deposition date:

2002-11-07

Original release date:

2003-04-10

Authors:

Gelis, Ioannis; Katsaros, Nikolaos; Luchinat, Claudio; Piccioli, Mario; Poggi, Luisa

Citation:

Citation: Gelis, Ioannis; Katsaros, Nikolaos; Luchinat, Claudio; Piccioli, Mario; Poggi, Luisa. "A Simple Protocol to study Blue Copper Proteins by NMR" Eur. J. Biochem. 270, 600-609 (2003).
PubMed: 12581200

Assembly members:

Assembly members:
Plastocyanin, polymer, 98 residues, Formula weight is not available
CU, non-polymer, 63.546 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 1148 Superkingdom: Eubacteria Kingdom: not available Genus/species: Synechocystis sp. PCC 6803

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Plastocyanin: ANATVKMGSDSGALVFEPST VTIKAGEEVKWVNNKLSPHN IVFAADGVDADTAAKLSHKG LAFAAGESFTSTFTEPGTYT YYCEPHRGAGMVGKVVVD

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	224

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	plastocyanin monomer	1
2	copper ion	2

Entities:

Entity 1, plastocyanin monomer 98 residues - Formula weight is not available

1

ALA

ASN

ALA

THR

VAL

LYS

MET

GLY

SER

ASP

2

SER

GLY

ALA

LEU

VAL

PHE

GLU

PRO

SER

THR

3

VAL

THR

ILE

LYS

ALA

GLY

GLU

VAL

LYS

4

TRP

VAL

ASN

LYS

LEU

SER

PRO

HIS

ASN

5

ILE

VAL

PHE

ALA

ASP

GLY

VAL

ASP

ALA

6

ASP

THR

ALA

LYS

LEU

SER

HIS

LYS

GLY

7

LEU

ALA

PHE

ALA

GLY

GLU

SER

PHE

THR

8

SER

THR

PHE

THR

GLU

PRO

GLY

THR

TYR

THR

9

TYR

CYS

GLU

PRO

HIS

ARG

GLY

ALA

GLY

10

MET

VAL

GLY

LYS

VAL

ASP

Entity 2, copper ion - Cu - 63.546 Da.

1

CU

Samples:

sample_1: Plastocyanin, [U-13C; U-15N], 2.0 mM

Ex-cond_1: pH: 5.2 na; temperature: 295 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	sample_1	not available	Ex-cond_1
1H-13C HSQC	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
CBCANH	sample_1	not available	Ex-cond_1
HNCO	sample_1	not available	Ex-cond_1

Software:

Sparky v3 - spectra assignment

Xwinnmr v3.1 - data processing

NMR spectrometers:

Bruker Avance 700 MHz
Bruker Avance 800 MHz

BMRB	5475
PDB	1J5C 1J5D 1JXD 1JXF 1M9W
DBJ	BAA10227 BAK51011 BAL30009 BAL33178 BAL36347
EMBL	CAA38038
GB	AGF52520
REF	NP_442157 WP_010873453 YP_005384024 YP_005387193 YP_005409900
SP	P21697
AlphaFold	P21697