BMRB Entry 5558

Name: Sp100b SAND domain chemical shift assignments
Published: 2002-12-27

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PDB ID: 1h5p
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5558
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sp100b SAND domain chemical shift assignments

Deposition date:

2002-10-15

Original release date:

2002-12-27

Authors:

Bottomley, Matthew; Collard, Michael; Huggenvik, Jodi; Liu, Zhihong; Gibson, Toby; Sattler, Michael

Citation:

Citation: Bottomley, Matthew; Collard, Michael; Huggenvik, Jodi; Liu, Zhihong; Gibson, Toby; Sattler, Michael. "The SAND domain structure defines a novel DNA-binding fold in transcriptional regulation" Nat. Struct. Biol. 8, 626-633 (2001).
PubMed: 11427895

Assembly members:

Assembly members:
SAND domain from human Sp100b protein, polymer, 104 residues, 12212 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-9d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SAND domain from human Sp100b protein: MKHHHHHHPMDENINFKQSE LPVTCGEVKGTLYKERFKQG TSKKCIQSEDKKWFTPREFE IEGDRGASKNWKLSIRCGGY TLKVLMENKFLPEPPSTRKK VTIK

Data sets:

assigned_chemical_shifts
- shift_set_1
coupling_constants
- J_values_set_1

Data type	Count
1H chemical shifts	485
13C chemical shifts	296
15N chemical shifts	97
coupling constants	30

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Sp100b SAND domain	1

Entities:

Entity 1, Sp100b SAND domain 104 residues - 12212 Da.

1

MET

LYS

HIS

PRO

MET

2

ASP

GLU

ASN

ILE

ASN

PHE

LYS

GLN

SER

GLU

3

LEU

PRO

VAL

THR

CYS

GLY

GLU

VAL

LYS

GLY

4

THR

LEU

TYR

LYS

GLU

ARG

PHE

LYS

GLN

GLY

5

THR

SER

LYS

CYS

ILE

GLN

SER

GLU

ASP

6

LYS

TRP

PHE

THR

PRO

ARG

GLU

PHE

GLU

7

ILE

GLU

GLY

ASP

ARG

GLY

ALA

SER

LYS

ASN

8

TRP

LYS

LEU

SER

ILE

ARG

CYS

GLY

TYR

9

THR

LEU

LYS

VAL

LEU

MET

GLU

ASN

LYS

PHE

10

LEU

PRO

GLU

PRO

SER

THR

ARG

LYS

11

VAL

THR

ILE

LYS

Samples:

Sample_1: SAND domain from human Sp100b protein, [U-95% 15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 4 mM

Sample_2: SAND domain from human Sp100b protein, [U-95% 13C; U-15N], 1.2 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DTT 4 mM

Sample_condition_1: pH: 6.5; temperature: 295 K; ionic strength: 0.07 M

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
CBCANH	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
H(C)CH TOCSY	not available	not available	not available
CCH TOCSY	not available	not available	not available
15N-edited NOESY	not available	not available	not available
13C-edited NOESY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DRX 500 MHz
Bruker DRX 600 MHz
Bruker DRX 700 MHz

PDB	1H5P
EMBL	CAH18143
GB	AAC50743 AAY14879 EAW70929
REF	NP_001193630 XP_004033374 XP_009442793 XP_009442794