BMRB Entry 5550

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5550

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Title:
Structure and Dynamics of Micelle-bound Neuropeptide Y: Comparison with unligated NPY and Implications for Receptor Selection
Deposition date:
2002-10-07
Original release date:
2008-07-17
Authors:
Bader, R.; Bettio, A.; Folkers, G.; Beck-Sickinger, A.G.; Zerbe, O.
Citation:

Citation: Bader, R.; Bettio, A.; Beck-Sickinger, A.G.; Zerbe, O.. "Structure and Dynamics of Micelle-bound Neuropeptide Y: Comparison with Unligated NPY and Implications for Receptor Selection"  J. Mol. Biol. 305, 307-329 (2001).
PubMed: 11124908

Assembly members:

Assembly members:
Neuropeptide Y, polymer, 37 residues, 4240 Da.

Natural source:

Natural source:   Common Name: Pig   Taxonomy ID: 9823   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Sus scrofa

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Neuropeptide Y: YPSKPDNPGEDAPAEDLARY YSALRHYINLITRQRYX

Data typeCount
1H chemical shifts260
15N chemical shifts64
T1 relaxation values93
T2 relaxation values112
heteronuclear NOE values62
order parameters27

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Neuropeptide Y1

Entities:

Entity 1, Neuropeptide Y 37 residues - 4240 Da.

1   TYRPROSERLYSPROASPASNPROGLYGLU
2   ASPALAPROALAGLUASPLEUALAARGTYR
3   TYRSERALALEUARGHISTYRILEASNLEU
4   ILETHRARGGLNARGTYRNH2

Samples:

sample_1: Neuropeptide Y, [U-95% 15N], 3 mM; DPC 0.30 M; H2O 90%; D2O 10%

sample_2: Neuropeptide Y, [U-95% 15N], 1 mM; H2O 90%; D2O 10%

Condition_1: pH: 6.0; temperature: 310 K; ionic strength: 0.15 M

Condition_2: pH: 3.1; temperature: 305 K; ionic strength: 0.15 M

Experiments:

NameSampleSample stateSample conditions
TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

X-PLOR v3.0 - structure calculation

NMR spectrometers:

  • Varian UnityPlus 500 MHz
  • Bruker AVANCE 600 MHz

Related Database Links:

BMRB 548
PDB
DBJ BAB22495 BAG35090 BAG82945 BAJ20461
EMBL CAD10677
GB AAA41722 AAA41723 AAA41724 AAA59944 AAA59946
PRF 1006203A 1407279B 1612231A 1907264A
REF NP_000896 NP_001009452 NP_001014845 NP_001027986 NP_001153758
SP P01303 P01304 P07808 P14765 P57774
TPG DAA30542
AlphaFold P01303 P01304 P07808 P14765 P57774

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