BMRB Entry 5545

Title:
NMR assignment of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)
Deposition date:
2002-10-02
Original release date:
2003-03-18
Authors:
Headey, Stephen; Yao, Shenggen; Parker, Nigel; Kantharidis, Phillip; Bach, Leon; Norton, Raymond
Citation:

Citation: Headey, Stephen; Yao, Shenggen; Parker, Nigel; Kantharidis, Phillip; Bach, Leon; Norton, Raymond. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)"  J. Biomol. NMR 25, 251-252 (2003).

Assembly members:

Assembly members:
insulin-like growth factor binding protein-6, polymer, 107 residues, 12056 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pProEX HTb

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts366
15N chemical shifts98
1H chemical shifts597

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1161-240 IGFBP-61

Entities:

Entity 1, 161-240 IGFBP-6 107 residues - 12056 Da.

1   SERTYRTYRHISHISHISHISHISHISASP
2   TYRASPILEPROTHRTHRGLUASNLEUTYR
3   PHEGLNGLYALAMETGLYSERGLYPROCYS
4   ARGARGHISLEUASPSERVALLEUGLNGLN
5   LEUGLNTHRGLUVALTYRARGGLYALAGLN
6   THRLEUTYRVALPROASNCYSASPHISARG
7   GLYPHETYRARGLYSARGGLNCYSARGSER
8   SERGLNGLYGLNARGARGGLYPROCYSTRP
9   CYSVALASPARGMETGLYLYSSERLEUPRO
10   GLYSERPROASPGLYASNGLYSERSERSER
11   CYSPROTHRGLYSERSERGLY

Samples:

single-labelled_sample: insulin-like growth factor binding protein-6, [U-50% 15N], 1.0 mM; Na acetate 10 mM; azide 0.02%; D2O 5%

double-labelled_sample: insulin-like growth factor binding protein-6, [U-95% 13C; U-50% 15N], 1.0 mM; Na acetate 10 mM; azide 0.02%; D2O 5%

Ex-cond_1: ionic strength: 0.01 M; pH: 4.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNHAnot availablenot availablenot available
HAHB(CO)NHnot availablenot availablenot available
1H-13C NOESYnot availablenot availablenot available

Software:

XWINNMR v3.1 -

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAG35976
EMBL CAA07346 CAG46590
GenBank AAA88070 AAB06187 AAH03507 AAH05007 AAH10162
REF NP_002169 XP_001087498 XP_509089
SWISS-PROT P24592

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks