BMRB Entry 5545

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PDB ID: 1rmj
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5545
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR assignment of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)

Deposition date:

2002-10-02

Original release date:

2003-03-18

Authors:

Headey, Stephen; Yao, Shenggen; Parker, Nigel; Kantharidis, Phillip; Bach, Leon; Norton, Raymond

Citation:

Citation: Headey, Stephen; Yao, Shenggen; Parker, Nigel; Kantharidis, Phillip; Bach, Leon; Norton, Raymond. "Letter to the Editor: 1H, 13C and 15N resonance assignments of the C-terminal domain of insulin-like growth factor binding protein-6 (IGFBP-6)" J. Biomol. NMR 25, 251-252 (2003).

Assembly members:

Assembly members:
insulin-like growth factor binding protein-6, polymer, 107 residues, 12056 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pProEX HTb

Entity Sequences (FASTA):

Entity Sequences (FASTA):
insulin-like growth factor binding protein-6: SYYHHHHHHDYDIPTTENLY FQGAMGSGPCRRHLDSVLQQ LQTEVYRGAQTLYVPNCDHR GFYRKRQCRSSQGQRRGPCW CVDRMGKSLPGSPDGNGSSS CPTGSSG

Data sets:

assigned_chemical_shifts
- CBP6_shifts_1

Data type	Count
13C chemical shifts	366
15N chemical shifts	98
1H chemical shifts	597

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	161-240 IGFBP-6	1

Entities:

Entity 1, 161-240 IGFBP-6 107 residues - 12056 Da.

1

SER

TYR

HIS

ASP

2

TYR

ASP

ILE

PRO

THR

GLU

ASN

LEU

TYR

3

PHE

GLN

GLY

ALA

MET

GLY

SER

GLY

PRO

CYS

4

ARG

HIS

LEU

ASP

SER

VAL

LEU

GLN

5

LEU

GLN

THR

GLU

VAL

TYR

ARG

GLY

ALA

GLN

6

THR

LEU

TYR

VAL

PRO

ASN

CYS

ASP

HIS

ARG

7

GLY

PHE

TYR

ARG

LYS

ARG

GLN

CYS

ARG

SER

8

SER

GLN

GLY

GLN

ARG

GLY

PRO

CYS

TRP

9

CYS

VAL

ASP

ARG

MET

GLY

LYS

SER

LEU

PRO

10

GLY

SER

PRO

ASP

GLY

ASN

GLY

SER

11

CYS

PRO

THR

GLY

SER

GLY

Samples:

single-labelled_sample: insulin-like growth factor binding protein-6, [U-50% 15N], 1.0 mM; Na acetate 10 mM; azide 0.02%; D2O 5%

double-labelled_sample: insulin-like growth factor binding protein-6, [U-95% 13C; U-50% 15N], 1.0 mM; Na acetate 10 mM; azide 0.02%; D2O 5%

Ex-cond_1: ionic strength: 0.01 M; pH: 4.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY-HSQC	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
HNHA	not available	not available	not available
HAHB(CO)NH	not available	not available	not available
1H-13C NOESY	not available	not available	not available

Software:

XWINNMR v3.1 -

NMR spectrometers:

Bruker DRX 600 MHz

PDB	1RMJ
DBJ	BAG35976
EMBL	CAA07346 CAG46590
GenBank	AAA88070 AAB06187 AAH03507 AAH05007 AAH10162
REF	NP_002169 XP_001087498 XP_509089
SWISS-PROT	P24592