BMRB Entry 5541
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5541
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NMR-STAR v3 text file.
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Citation: Luecke, Christian; Kizilbash, Nadeem; van Moerkerk, Herman; Veerkamp, Jacques; Hamilton, James. "Letter to the Editor: NMR assignment and structural characterization of the
fatty acid binding protein from the flight muscle of Locusta migratoria" J. Biomol. NMR 25, 355-356 (2003).
Assembly members:
fatty acid binding protein, polymer, 134 residues, Formula weight is not available
Natural source: Common Name: Locust Taxonomy ID: 7004 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Locusta migratoria
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3d
Entity Sequences (FASTA):
fatty acid binding protein: MVKEFAGIKYKLDSQTNFEE
YMKAIGVGAIERKAGLALSP
VIELEVLDGDKFKLTSKTAI
KNTEFTFKLGEEFDEDTLDG
RKVKSIITQDGPNKLVHEQK
GDHPTIIIREFSKEQCVITI
KLGDLVATRIYKAQ
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 962 |
| 15N chemical shifts | 126 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Lm-FABP | 1 |
Entities:
Entity 1, Lm-FABP 134 residues - Formula weight is not available
| 1 | MET | VAL | LYS | GLU | PHE | ALA | GLY | ILE | LYS | TYR | ||||
| 2 | LYS | LEU | ASP | SER | GLN | THR | ASN | PHE | GLU | GLU | ||||
| 3 | TYR | MET | LYS | ALA | ILE | GLY | VAL | GLY | ALA | ILE | ||||
| 4 | GLU | ARG | LYS | ALA | GLY | LEU | ALA | LEU | SER | PRO | ||||
| 5 | VAL | ILE | GLU | LEU | GLU | VAL | LEU | ASP | GLY | ASP | ||||
| 6 | LYS | PHE | LYS | LEU | THR | SER | LYS | THR | ALA | ILE | ||||
| 7 | LYS | ASN | THR | GLU | PHE | THR | PHE | LYS | LEU | GLY | ||||
| 8 | GLU | GLU | PHE | ASP | GLU | ASP | THR | LEU | ASP | GLY | ||||
| 9 | ARG | LYS | VAL | LYS | SER | ILE | ILE | THR | GLN | ASP | ||||
| 10 | GLY | PRO | ASN | LYS | LEU | VAL | HIS | GLU | GLN | LYS | ||||
| 11 | GLY | ASP | HIS | PRO | THR | ILE | ILE | ILE | ARG | GLU | ||||
| 12 | PHE | SER | LYS | GLU | GLN | CYS | VAL | ILE | THR | ILE | ||||
| 13 | LYS | LEU | GLY | ASP | LEU | VAL | ALA | THR | ARG | ILE | ||||
| 14 | TYR | LYS | ALA | GLN |
Samples:
sample_1: fatty acid binding protein 5 mM
sample_2: fatty acid binding protein, [U-15N], 1 mM
cond_1: pH: 5.5; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY mixing times were set to 150ms (3D) and 200ms (2D); | not available | not available | cond_1 |
| TOCSY spinlock times of either 80ms or 6ms were used. | not available | not available | cond_1 |
Software:
XWINNMR v1.3 - processing
AURELIA v2.5.9 - analysis
NMR spectrometers:
- Bruker DMX 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks