BMRB Entry 5538

Name: 1H, 13C, and 15N Chemical Shift Assignments for a PWWP domain from Sch. pombe
Published: 2003-08-01

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PDB ID: 1h3z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5538
MolProbity Validation Chart

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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for a PWWP domain from Sch. pombe

Deposition date:

2002-09-25

Original release date:

2003-08-01

Authors:

Slater, Leanne; Allen, Mark; Bycroft, Mark

Citation:

Citation: Slater, Leanne; Allen, Mark; Bycroft, Mark. "Structural Variation in PWWP Domains" J. Mol. Biol. 330, 571-576 (2003).
PubMed: 12842472

Assembly members:

Assembly members:
PWWP domain, polymer, 109 residues, 12194 Da.

Natural source:

Natural source: Common Name: Fission yeast Taxonomy ID: 4896 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Schizosaccharomyces pombe

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PWWP domain: GSERVNYKPGMRVLTKMSGF PWWPSMVVTESKMTSVARKS KPKRAGTFYPVIFFPNKEYL WTGSDSLTPLTSEAISQFLE KPKPKTASLIKAYKMAQSTP DLDSLSVPS

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	787
13C chemical shifts	364
15N chemical shifts	104

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PWWP domain	1

Entities:

Entity 1, PWWP domain 109 residues - 12194 Da.

1

GLY

SER

GLU

ARG

VAL

ASN

TYR

LYS

PRO

GLY

2

MET

ARG

VAL

LEU

THR

LYS

MET

SER

GLY

PHE

3

PRO

TRP

PRO

SER

MET

VAL

THR

GLU

4

SER

LYS

MET

THR

SER

VAL

ALA

ARG

LYS

SER

5

LYS

PRO

LYS

ARG

ALA

GLY

THR

PHE

TYR

PRO

6

VAL

ILE

PHE

PRO

ASN

LYS

GLU

TYR

LEU

7

TRP

THR

GLY

SER

ASP

SER

LEU

THR

PRO

LEU

8

THR

SER

GLU

ALA

ILE

SER

GLN

PHE

LEU

GLU

9

LYS

PRO

LYS

PRO

LYS

THR

ALA

SER

LEU

ILE

10

LYS

ALA

TYR

LYS

MET

ALA

GLN

SER

THR

PRO

11

ASP

LEU

ASP

SER

LEU

SER

VAL

PRO

SER

Samples:

sample_1: PWWP domain, [U-99% 13C; U-99% 15N], 1.0 mM; potassium phosphate 50 mM; NaCl 300 mM

Ex-cond_1: pH: 6.0; temperature: 290 K; ionic strength: 0.35 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
2D DQF-COSY	not available	not available	not available
15N-HSQC	not available	not available	not available
13C-HSQC	not available	not available	not available
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCO	not available	not available	not available
HNHB	not available	not available	not available
13C-NOESY	not available	not available	not available

Software:

Ansig v3.3 - assignment, through space NOE determination

NMR spectrometers:

Bruker Advance 800 MHz
Bruker Advance 600 MHz

PDB	1H3Z
EMBL	CAA22121
REF	NP_596684
SP	O94312
AlphaFold	O94312