BMRB Entry 5515
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5515
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Citation: Kutateladze, Tatiana; Capelluto, Daniel; Overduin, Michael. "1H, 13C and 15N resonance assignment of the Dishevelled DIX domain" J. Biomol. NMR ., .-..
Assembly members:
Dvl2 Dix, polymer, 85 residues, Formula weight is not available
Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Dvl2 Dix: GVGETKVIYHLDEEETPYLV
KIPVPAERITLGDFKSVLQR
PAGAKYFFKSMDQDFGVVKE
EISDDNARLPCFNGRVVSWL
VSSDT
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 311 |
| 15N chemical shifts | 85 |
| 1H chemical shifts | 604 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Dix subunit 1 | 1 |
| 2 | Dix subunit 2 | 1 |
Entities:
Entity 1, Dix subunit 1 85 residues - Formula weight is not available
| 1 | GLY | VAL | GLY | GLU | THR | LYS | VAL | ILE | TYR | HIS | ||||
| 2 | LEU | ASP | GLU | GLU | GLU | THR | PRO | TYR | LEU | VAL | ||||
| 3 | LYS | ILE | PRO | VAL | PRO | ALA | GLU | ARG | ILE | THR | ||||
| 4 | LEU | GLY | ASP | PHE | LYS | SER | VAL | LEU | GLN | ARG | ||||
| 5 | PRO | ALA | GLY | ALA | LYS | TYR | PHE | PHE | LYS | SER | ||||
| 6 | MET | ASP | GLN | ASP | PHE | GLY | VAL | VAL | LYS | GLU | ||||
| 7 | GLU | ILE | SER | ASP | ASP | ASN | ALA | ARG | LEU | PRO | ||||
| 8 | CYS | PHE | ASN | GLY | ARG | VAL | VAL | SER | TRP | LEU | ||||
| 9 | VAL | SER | SER | ASP | THR |
Samples:
sample_1: Dvl2 Dix, [U-95% 13C; U-90% 15N], 1.0 mM
Ex-cond_1: pH: 6.5; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D TOCSY | not available | not available | not available |
| 2D NOESY | not available | not available | not available |
| 1H,15N-HSQC | not available | not available | not available |
| CBCA(CO)NNH | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNHA | not available | not available | not available |
| H(CCO)NH TOCSY | not available | not available | not available |
| C(CO)NH TOCSY | not available | not available | not available |
| 1H-15N TOCSY | not available | not available | not available |
| 1H-15N NOESY | not available | not available | not available |
| HSQC-NOESY-HSQC | not available | not available | not available |
| 1H-15N HMQCJ | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian INOVA 500 MHz
Related Database Links:
| DBJ | BAE20679 BAE27460 BAE35461 BAE40307 BAG59612 |
| GB | AAB65243 AAC52827 AAH14844 AAH53050 AAH92396 |
| REF | NP_001178311 NP_001181735 NP_004413 NP_031914 XP_001106375 |
| SP | O14641 Q60838 |
| TPG | DAA18923 |
| AlphaFold | O14641 Q60838 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks