BMRB Entry 5514

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5514

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone HN, N, Ca and Cb assignment of the GFPuv mutant, an 54 kDa protein
Deposition date:
2002-09-09
Original release date:
2003-02-18
Authors:
Georgescu, Julia; Rehm, Till; Wiehler, Jens; Steipe, Boris; Holak, Tad
Citation:

Citation: Georgescu, Julia; Rehm, Till; Wiehler, Jens; Steipe, Boris; Holak, Tad. "Letter to the editor: Backbone HN, N, Ca and Cb assignment of the GFPuv mutant"  J. Biomol. NMR 25, 161-162 (2003).

Assembly members:

Assembly members:
green fluorescent protein uv, polymer, 239 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Jellyfish   Taxonomy ID: 6100   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Aequorea victoria

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
green fluorescent protein uv: MGKGEELFTGVVPILVELDG DVNGHKFSVSGEGEGDATYG KLTLKFICTTGKLPVPWPTL VTTFSYGVQCFSRYPDHMKR HDFFKSAMPEGYVQERTISF KDDGNYKTRAEVKFEGDTLV NRIELKGIDFKEDGNILGHK LEYNYNSHNVYITADKQKNG IKANFKIRHNIEDGSVQLAD HYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVL LEFVTAAGITHGMDELYKG

Data sets:
Data typeCount
1H chemical shifts319
13C chemical shifts536
15N chemical shifts184

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1GFPuv mutant, chain A1
2GFPuv mutant, chain B1

Entities:

Entity 1, GFPuv mutant, chain A 239 residues - Formula weight is not available

1   METGLYLYSGLYGLUGLULEUPHETHRGLY
2   VALVALPROILELEUVALGLULEUASPGLY
3   ASPVALASNGLYHISLYSPHESERVALSER
4   GLYGLUGLYGLUGLYASPALATHRTYRGLY
5   LYSLEUTHRLEULYSPHEILECYSTHRTHR
6   GLYLYSLEUPROVALPROTRPPROTHRLEU
7   VALTHRTHRPHESERTYRGLYVALGLNCYS
8   PHESERARGTYRPROASPHISMETLYSARG
9   HISASPPHEPHELYSSERALAMETPROGLU
10   GLYTYRVALGLNGLUARGTHRILESERPHE
11   LYSASPASPGLYASNTYRLYSTHRARGALA
12   GLUVALLYSPHEGLUGLYASPTHRLEUVAL
13   ASNARGILEGLULEULYSGLYILEASPPHE
14   LYSGLUASPGLYASNILELEUGLYHISLYS
15   LEUGLUTYRASNTYRASNSERHISASNVAL
16   TYRILETHRALAASPLYSGLNLYSASNGLY
17   ILELYSALAASNPHELYSILEARGHISASN
18   ILEGLUASPGLYSERVALGLNLEUALAASP
19   HISTYRGLNGLNASNTHRPROILEGLYASP
20   GLYPROVALLEULEUPROASPASNHISTYR
21   LEUSERTHRGLNSERALALEUSERLYSASP
22   PROASNGLULYSARGASPHISMETVALLEU
23   LEUGLUPHEVALTHRALAALAGLYILETHR
24   HISGLYMETASPGLULEUTYRLYSGLY

Samples:

sample1: green fluorescent protein uv, [U-15N], 0.8 – 1.5 mM

sample2: green fluorescent protein uv, [U-15N; U-13C; U-70% 2H], 0.8 – 1.5 mM

Ex-cond: pH: 7.0 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY HSQCnot availablenot availablenot available
2D-TOCSYnot availablenot availablenot available
2D NOESY in D2Onot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available

Software:

ccnmr-glxcc - processing, assignment

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

BMRB 5666
PDB
DBJ BAA93576 BAB32740 BAB68313 BAB68314 BAB72232
EMBL CAA10279 CAA65278 CAB61435 CAB70975 CAC27829
GB AAA27721 AAA69542 AAA69544 AAA69546 AAA69548
REF WP_006462513 WP_012569506 YP_002302326
SP P42212
AlphaFold P42212

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks