BMRB Entry 5503
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5503
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Matei, E.; Miron, S.; Blouquit, Y.; Duchambon, P.; Durussel, P.; Cox, J.; Craescu, C.. "C-terminal Half of Human Centrin 2 Behaves like a Regulatory EF-hand Domain" Biochemistry 42, 1439-1450 (2003).
PubMed: 12578356
Assembly members:
Human centrin2, polymer, 89 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET24a(+)
Entity Sequences (FASTA):
Human centrin2: MNFGDFLTVMTQKMSEKDTK
EEILKAFKLFDDDETGKISF
KNLKRVAKELGENLTDEELQ
EMIDEADRDGDGEVSEQEFL
RIMKKTSLY
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 540 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Human centrin2 | 1 |
Entities:
Entity 1, Human centrin2 89 residues - Formula weight is not available
| 1 | MET | ASN | PHE | GLY | ASP | PHE | LEU | THR | VAL | MET | ||||
| 2 | THR | GLN | LYS | MET | SER | GLU | LYS | ASP | THR | LYS | ||||
| 3 | GLU | GLU | ILE | LEU | LYS | ALA | PHE | LYS | LEU | PHE | ||||
| 4 | ASP | ASP | ASP | GLU | THR | GLY | LYS | ILE | SER | PHE | ||||
| 5 | LYS | ASN | LEU | LYS | ARG | VAL | ALA | LYS | GLU | LEU | ||||
| 6 | GLY | GLU | ASN | LEU | THR | ASP | GLU | GLU | LEU | GLN | ||||
| 7 | GLU | MET | ILE | ASP | GLU | ALA | ASP | ARG | ASP | GLY | ||||
| 8 | ASP | GLY | GLU | VAL | SER | GLU | GLN | GLU | PHE | LEU | ||||
| 9 | ARG | ILE | MET | LYS | LYS | THR | SER | LEU | TYR |
Samples:
sample_one: Human centrin2 1.5 mM; Tris_d11, [U-2H], 20 mM; NaCl 100 mM; H2O 95%; D2O 5%
sample_two: Human centrin2 1.4 mM; Tris_d11, [U-2H], 20 mM; NaCl 100 mM; H2O 95%; D2O 5%
sample_cond_1: pH: 6.5; temperature: 308 K; ionic strength: 100 mM; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | not available | not available | not available |
| 2D TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
Software:
DGII vI2000 - structure solution
DISCOVER vI2000 - refinement
NMR spectrometers:
- Varian UNITY 500 MHz