BMRB Entry 5499

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PDB ID: 1m5z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5499
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area

Deposition date:

2002-08-13

Original release date:

2003-02-20

Authors:

Feng, Wei; Fan, Jing-Song; Jiang, Ming; Shi, Ya-Wei; Zhang, Mingjie

Citation:

Citation: Feng, Wei; Fan, Jing-Song; Jiang, Ming; Shi, Ya-Wei; Zhang, Mingjie. "PDZ7 of Glutamate Receptor Interacting Protein Binds to its Target via a Novel Hydrophobic Surface Area" J. Biol. Chem. 277, 41140-41146 (2002).
PubMed: 12196542

Assembly members:

Assembly members:
the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, polymer, 91 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Norway rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
the seventh PDZ domain of Glutamate Receptor Interacting Protein 1: SPTPVELHKVTLYKDSGMED FGFSVADGLLEKGVYVKNIR PAGPGDLGGLKPYDRLLQVN HVRTRDFDCCLVVPLIAESG NKLDLVISRNP

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	584
13C chemical shifts	369
15N chemical shifts	84

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	AMPA receptor interacting protein	1

Entities:

Entity 1, AMPA receptor interacting protein 91 residues - Formula weight is not available

1

SER

PRO

THR

PRO

VAL

GLU

LEU

HIS

LYS

VAL

2

THR

LEU

TYR

LYS

ASP

SER

GLY

MET

GLU

ASP

3

PHE

GLY

PHE

SER

VAL

ALA

ASP

GLY

LEU

4

GLU

LYS

GLY

VAL

TYR

VAL

LYS

ASN

ILE

ARG

5

PRO

ALA

GLY

PRO

GLY

ASP

LEU

GLY

LEU

6

LYS

PRO

TYR

ASP

ARG

LEU

GLN

VAL

ASN

7

HIS

VAL

ARG

THR

ARG

ASP

PHE

ASP

CYS

8

LEU

VAL

PRO

LEU

ILE

ALA

GLU

SER

GLY

9

ASN

LYS

LEU

ASP

LEU

VAL

ILE

SER

ARG

ASN

10

PRO

Samples:

sample_1: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1 1.0 mM; D2O 99.9%; potassium phosphate buffer 100 mM

sample_2: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, [U-100% 15N], 1 mM; H2O 90%; D20 10%; potassium phosphate buffer 100 mM

sample_3: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, [U-100% 15N;U-100% 13C], 1.0 mM; D2O 99.9%; potassium phosphate buffer 100 mM

sample_4: the seventh PDZ domain of Glutamate Receptor Interacting Protein 1, [U-100% 15N; U-100% 13C], 1.0 mM; H2O 90%; D2O 10%; potassium phosphate buffer 100 mM

sample_cond_1: pH: 6.5 na; temperature: 298 K; ionic strength: 100 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
3D 15N-separated_NOESY	not available	not available	not available
3D 13C-separated_NOESY	not available	not available	not available

Software:

CNS v1.1 - structure solution

NMR spectrometers:

Varian INOVA 750 MHz

PDB	1M5Z
DBJ	BAC26668
EMBL	CAB39895
GB	AAP81257 AAQ56581 AAR08916 ABB46288 EDL24394
REF	NP_001264224 XP_002687693 XP_004007495 XP_004330097 XP_005962414
TPG	DAA29720