BMRB Entry 5492

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5492

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H and 15N Chemical Shift Assignments for the charge reverse variant of Ribonuclease Sa "5K" (D1K, D17K, D25K, E41K, E74K)

Deposition date:

2002-08-05

Original release date:

2003-02-20

Authors:

Laurents, Douglas; Huyghues-Despointes, Beatrice; Bruix, Marta; Thurlkill, Richard; Schell, David; Newsome, Stephanie; Grimsley, Gerald; Shaw, Kevin; Trevino, Saul; Rico, Manuel; Scholtz, J. Martin; Pace, C. Nick

Citation:

Citation: Laurents, Douglas; Huyghues-Despointes, Beatrice; Bruix, Marta; Thurlkill, Richard; Schell, David; Newsom, Stephanie; Grimsley, Gerald; Shaw, Kevin; Trevino, Saul; Rico, Manuel; Briggs, James; Antosiewicz, Jan; Scholtz, J.; Pace, C.. "Charge-Charge Interactions are Key Determinants of the pK Values of Ionizable Groups in Ribonuclease Sa (pI=3.5) and a Basic Variant (pI=10.2)" J. Mol. Biol. 325, 1077-1092 (2003).
PubMed: 12527309

Assembly members:

Assembly members:
Ribonuclease Sa, polymer, 96 residues, Formula weight is not available

Natural source:

Natural source: Common Name: S. aureofaciens Taxonomy ID: 1894 Superkingdom: Eubacteria Kingdom: not available Genus/species: Streptomyces aureofaciens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribonuclease Sa: KVSGTVCLSALPPEATKTLN LIASKGPFPYSQDGVVFQNR KSVLPTQSYGYYHEYTVITP GARTRGTRRIITGKATQEDY YTGDHYATFSLIDQTC

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	489
15N chemical shifts	89

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	5K RNase Sa	1

Entities:

Entity 1, 5K RNase Sa 96 residues - Formula weight is not available

1

LYS

VAL

SER

GLY

THR

VAL

CYS

LEU

SER

ALA

2

LEU

PRO

GLU

ALA

THR

LYS

THR

LEU

ASN

3

LEU

ILE

ALA

SER

LYS

GLY

PRO

PHE

PRO

TYR

4

SER

GLN

ASP

GLY

VAL

PHE

GLN

ASN

ARG

5

LYS

SER

VAL

LEU

PRO

THR

GLN

SER

TYR

GLY

6

TYR

HIS

GLU

TYR

THR

VAL

ILE

THR

PRO

7

GLY

ALA

ARG

THR

ARG

GLY

THR

ARG

ILE

8

ILE

THR

GLY

LYS

ALA

THR

GLN

GLU

ASP

TYR

9

TYR

THR

GLY

ASP

HIS

TYR

ALA

THR

PHE

SER

10

LEU

ILE

ASP

GLN

THR

CYS

Samples:

sample_1: Ribonuclease Sa, [U-13C; U-15N], 1 – 2 mM; NaCl 0.005 M

Ex_cond_1: pH: 5.5; temperature: 303 K; ionic strength: 0.005 M

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	not available	not available	not available
2D 1H-1H NOESY	not available	not available	not available
2D 1H-15N HSQC	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker AMX 600 MHz