BMRB Entry 5484
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5484
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lee, Yeon-Hee; Won, Hyung-Sik; Ahn, Hee-Chul; Park, Sangho; Yagi, H.; Akutsu, H.; Lee, Bong-Jin. "Letter to the Editor: Backbone NMR assignments of the metal-free UreE from
Bacillus pasteurii" J. Biomol. NMR 24, 361-362 (2002).
Assembly members:
Bacillus pasteurii UreE, polymer, 147 residues, 34768 Da.
Natural source: Common Name: Bacillus pasteurii Taxonomy ID: 1474 Superkingdom: Eubacteria Kingdom: not available Genus/species: Bacillus pasteurii
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Bacillus pasteurii UreE: MLITKIVGHIDDYESSDKKV
DWLEVEWEDLNKRILRKETE
NGTDIAIKLENSGTLRYGDV
LYESDDTLIAIRTKLEKVYV
IKPQTMQEMGKMAFEIGNRH
TMCIIEDDEILVRYDKTLEK
LIDEVGVSYEQSERRFKEPF
KYRGHQH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 433 |
| 15N chemical shifts | 142 |
| 1H chemical shifts | 142 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BpUreE subunit 1 | 1 |
| 2 | BpUreE subunit 2 | 1 |
Entities:
Entity 1, BpUreE subunit 1 147 residues - 34768 Da.
| 1 | MET | LEU | ILE | THR | LYS | ILE | VAL | GLY | HIS | ILE | ||||
| 2 | ASP | ASP | TYR | GLU | SER | SER | ASP | LYS | LYS | VAL | ||||
| 3 | ASP | TRP | LEU | GLU | VAL | GLU | TRP | GLU | ASP | LEU | ||||
| 4 | ASN | LYS | ARG | ILE | LEU | ARG | LYS | GLU | THR | GLU | ||||
| 5 | ASN | GLY | THR | ASP | ILE | ALA | ILE | LYS | LEU | GLU | ||||
| 6 | ASN | SER | GLY | THR | LEU | ARG | TYR | GLY | ASP | VAL | ||||
| 7 | LEU | TYR | GLU | SER | ASP | ASP | THR | LEU | ILE | ALA | ||||
| 8 | ILE | ARG | THR | LYS | LEU | GLU | LYS | VAL | TYR | VAL | ||||
| 9 | ILE | LYS | PRO | GLN | THR | MET | GLN | GLU | MET | GLY | ||||
| 10 | LYS | MET | ALA | PHE | GLU | ILE | GLY | ASN | ARG | HIS | ||||
| 11 | THR | MET | CYS | ILE | ILE | GLU | ASP | ASP | GLU | ILE | ||||
| 12 | LEU | VAL | ARG | TYR | ASP | LYS | THR | LEU | GLU | LYS | ||||
| 13 | LEU | ILE | ASP | GLU | VAL | GLY | VAL | SER | TYR | GLU | ||||
| 14 | GLN | SER | GLU | ARG | ARG | PHE | LYS | GLU | PRO | PHE | ||||
| 15 | LYS | TYR | ARG | GLY | HIS | GLN | HIS |
Samples:
sample_1: Bacillus pasteurii UreE, [U-90% 2H; U-13C; U-15N], 1.4 mM
Ex-cond_1: pH: 6.5; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| [1H/15N]HSQC | not available | not available | not available |
| 15N-edited NOESY-HSQC | not available | not available | not available |
| HNCA | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HN(CA)CB | not available | not available | not available |
| HN(COCA)CB | not available | not available | not available |
| HNCO | not available | not available | not available |
| HNCACO | not available | not available | not available |
Software:
NMRPipe -
NMRView -
NMR spectrometers:
- Bruker DRX 600 MHz
- Bruker DRX 800 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks