BMRB Entry 5481

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PDB ID: 1m94
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5481
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side chain 1H, 13C, and 15N chemical shift assignments for S. cerevisae Hub1

Deposition date:

2002-07-25

Original release date:

2003-07-30

Authors:

Ramelot, Theresa; Kennedy, Michael

Citation:

Citation: Ramelot, Theresa; Cort, R.; Yee, A.; Semesi, A.; Edwards, A.; Arrowsmith, C.; Kennedy, Michael. "Solution structure of the yeast ubiquitin-like modifier protein Hub1" J. Struct. Funct. Genomics 4, 25-30 (2003).

Assembly members:

Assembly members:
Hub1, polymer, 93 residues, 8272 Da.

Natural source:

Natural source: Common Name: Yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Hub1: MGSSHHHHHHSSGLVPRGSH MIEVVVNDRLGKKVRVKCLA EDSVGDFKKVLSLQIGTQPN KIVLQKGGSVLKDHISLEDY EVHDQTNLELYYL

Data sets:

assigned_chemical_shifts
- hub1_shifts

Data type	Count
1H chemical shifts	537
13C chemical shifts	321
15N chemical shifts	75

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hub1	1

Entities:

Entity 1, hub1 93 residues - 8272 Da.

1

MET

GLY

SER

HIS

2

SER

GLY

LEU

VAL

PRO

ARG

GLY

SER

HIS

3

MET

ILE

GLU

VAL

ASN

ASP

ARG

LEU

4

GLY

LYS

VAL

ARG

VAL

LYS

CYS

LEU

ALA

5

GLU

ASP

SER

VAL

GLY

ASP

PHE

LYS

VAL

6

LEU

SER

LEU

GLN

ILE

GLY

THR

GLN

PRO

ASN

7

LYS

ILE

VAL

LEU

GLN

LYS

GLY

SER

VAL

8

LEU

LYS

ASP

HIS

ILE

SER

LEU

GLU

ASP

TYR

9

GLU

VAL

HIS

ASP

GLN

THR

ASN

LEU

GLU

LEU

10

TYR

LEU

Samples:

sample_1: Hub1, [U-15N; U-13C], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM

sample_2: Hub1, [U-15N], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM

sample_3: Hub1, [U-10% 13C; U-15N], 1 – 3 mM; acetate buffer 10 mM; NaCl 300 mM

cond_1: pH: 5.0; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
4D CC-NOESY and 1H-15N HSQC spectra were recorded after exchange into D2O.	not available	not available	cond_1
1H-13C HSQC was recorded on U-15N, 10% 13C labeled sample	not available	not available	cond_1

Software:

Felix v98 - processing data

XPLOR v3.84 - structure calculations

Sparky v3.98 - chemical shift and noesy assignments

NMR spectrometers:

Varian Inova 600 MHz
Varian Inova 750 MHz
Varian Inova 800 MHz

PDB	1M94 3PLU 3PLV
DBJ	GAA26125
EMBL	CAY82226
GB	AAS56885 AHY77119 EDN62838 EDV12235 EEU08318
PIR	S78735
REF	NP_014430
SP	Q6Q546
TPG	DAA10573
AlphaFold	Q6Q546