BMRB Entry 5471

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5471

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Title:
Backbone HN,13C,15N assignments for Malate Synthase G from E.coli
Deposition date:
2002-07-23
Original release date:
2002-09-05
Authors:
Tugarinov, Vitali; Muhandiram, Ranjith; Ayed, Ayeda; Kay, Lewis
Citation:

Citation: Tugarinov, Vitali; Muhandiram, Ranjith; Ayed, Ayeda; Kay, Lewis. "Four-dimensional NMR Spectroscopy of a 723-residue Protein: Chemical Shift Assignments and Secondary Structure of Malate Synthase G"  J. Am. Chem. Soc. 124, 10025-10035 (2002).

Assembly members:

Assembly members:
malate synthase G, polymer, 731 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
malate synthase G: MAQTITQSRLRIDANFKRFV DEEVLPGTGLDAAAFWRNFD EIVHDLAPENRQLLAERDRI QAALDEWHRSNPGPVKDKAA YKSFLRELGYLVPQPERVTV ETTGIDSEITSQAGPQLVVP AMNARYALNAANARWGSLYD ALYGSDIIPQEGAMVSGYDP QRGEQVIAWVRRFLDESLPL ENGSYQDVVAFKVVDKQLRI QLKNGKETTLRTPAQFVGYR GDAAAPTCILLKNNGLHIEL QIDANGRIGKDDPAHINDVI VEAAISTILDCEDSVAAVDA EDKILLYRNLLGLMQGTLQE KMEKNGRQIVRKLNDDRHYT AADGSEISLHGRSLLFIRNV GHLMTIPVIWDSEGNEIPEG ILDGVMTGAIALYDLKVQKN SRTGSVYIVKPKMHGPQEVA FANKLFTRIETMLGMAPNTL KMGIMDEERRTSLNLRSCIA QARNRVAFINTGFLDRTGDE MHSVMEAGPMLRKNQMKSTP WIKAYERNNVLSGLFCGLRG KAQIGKGMWAMPDLMADMYS QKGDQLRAGANTAWVPSPTA ATLHALHYHQTNVQSVQANI AQTEFNAEFEPLLDDLLTIP VAENANWSAQEIQQELDNNV QGILGYVVRWVEQGIGCSKV PDIHNVALMEDRATLRISSQ HIANWLRHGILTKEQVQASL ENMAKVVDQQNAGDPAYRPM AGNFANSCAFKAASDLIFLG VKQPNGYTEPLLHAWRLREK ESHLEHHHHHH

Data sets:
Data typeCount
1H chemical shifts654
13C chemical shifts2046
15N chemical shifts654

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1malate synthase G1

Entities:

Entity 1, malate synthase G 731 residues - Formula weight is not available

1   METALAGLNTHRILETHRGLNSERARGLEU
2   ARGILEASPALAASNPHELYSARGPHEVAL
3   ASPGLUGLUVALLEUPROGLYTHRGLYLEU
4   ASPALAALAALAPHETRPARGASNPHEASP
5   GLUILEVALHISASPLEUALAPROGLUASN
6   ARGGLNLEULEUALAGLUARGASPARGILE
7   GLNALAALALEUASPGLUTRPHISARGSER
8   ASNPROGLYPROVALLYSASPLYSALAALA
9   TYRLYSSERPHELEUARGGLULEUGLYTYR
10   LEUVALPROGLNPROGLUARGVALTHRVAL
11   GLUTHRTHRGLYILEASPSERGLUILETHR
12   SERGLNALAGLYPROGLNLEUVALVALPRO
13   ALAMETASNALAARGTYRALALEUASNALA
14   ALAASNALAARGTRPGLYSERLEUTYRASP
15   ALALEUTYRGLYSERASPILEILEPROGLN
16   GLUGLYALAMETVALSERGLYTYRASPPRO
17   GLNARGGLYGLUGLNVALILEALATRPVAL
18   ARGARGPHELEUASPGLUSERLEUPROLEU
19   GLUASNGLYSERTYRGLNASPVALVALALA
20   PHELYSVALVALASPLYSGLNLEUARGILE
21   GLNLEULYSASNGLYLYSGLUTHRTHRLEU
22   ARGTHRPROALAGLNPHEVALGLYTYRARG
23   GLYASPALAALAALAPROTHRCYSILELEU
24   LEULYSASNASNGLYLEUHISILEGLULEU
25   GLNILEASPALAASNGLYARGILEGLYLYS
26   ASPASPPROALAHISILEASNASPVALILE
27   VALGLUALAALAILESERTHRILELEUASP
28   CYSGLUASPSERVALALAALAVALASPALA
29   GLUASPLYSILELEULEUTYRARGASNLEU
30   LEUGLYLEUMETGLNGLYTHRLEUGLNGLU
31   LYSMETGLULYSASNGLYARGGLNILEVAL
32   ARGLYSLEUASNASPASPARGHISTYRTHR
33   ALAALAASPGLYSERGLUILESERLEUHIS
34   GLYARGSERLEULEUPHEILEARGASNVAL
35   GLYHISLEUMETTHRILEPROVALILETRP
36   ASPSERGLUGLYASNGLUILEPROGLUGLY
37   ILELEUASPGLYVALMETTHRGLYALAILE
38   ALALEUTYRASPLEULYSVALGLNLYSASN
39   SERARGTHRGLYSERVALTYRILEVALLYS
40   PROLYSMETHISGLYPROGLNGLUVALALA
41   PHEALAASNLYSLEUPHETHRARGILEGLU
42   THRMETLEUGLYMETALAPROASNTHRLEU
43   LYSMETGLYILEMETASPGLUGLUARGARG
44   THRSERLEUASNLEUARGSERCYSILEALA
45   GLNALAARGASNARGVALALAPHEILEASN
46   THRGLYPHELEUASPARGTHRGLYASPGLU
47   METHISSERVALMETGLUALAGLYPROMET
48   LEUARGLYSASNGLNMETLYSSERTHRPRO
49   TRPILELYSALATYRGLUARGASNASNVAL
50   LEUSERGLYLEUPHECYSGLYLEUARGGLY
51   LYSALAGLNILEGLYLYSGLYMETTRPALA
52   METPROASPLEUMETALAASPMETTYRSER
53   GLNLYSGLYASPGLNLEUARGALAGLYALA
54   ASNTHRALATRPVALPROSERPROTHRALA
55   ALATHRLEUHISALALEUHISTYRHISGLN
56   THRASNVALGLNSERVALGLNALAASNILE
57   ALAGLNTHRGLUPHEASNALAGLUPHEGLU
58   PROLEULEUASPASPLEULEUTHRILEPRO
59   VALALAGLUASNALAASNTRPSERALAGLN
60   GLUILEGLNGLNGLULEUASPASNASNVAL
61   GLNGLYILELEUGLYTYRVALVALARGTRP
62   VALGLUGLNGLYILEGLYCYSSERLYSVAL
63   PROASPILEHISASNVALALALEUMETGLU
64   ASPARGALATHRLEUARGILESERSERGLN
65   HISILEALAASNTRPLEUARGHISGLYILE
66   LEUTHRLYSGLUGLNVALGLNALASERLEU
67   GLUASNMETALALYSVALVALASPGLNGLN
68   ASNALAGLYASPPROALATYRARGPROMET
69   ALAGLYASNPHEALAASNSERCYSALAPHE
70   LYSALAALASERASPLEUILEPHELEUGLY
71   VALLYSGLNPROASNGLYTYRTHRGLUPRO
72   LEULEUHISALATRPARGLEUARGGLULYS
73   GLUSERHISLEUGLUHISHISHISHISHIS
74   HIS

Samples:

sample_1: malate synthase G, [U-13C; U-15N; U-2H], 0.5 – 0.8 mM; MgCl2 20 mM

sample_2: malate synthase G, [U-15N]; [U-2H; NA-Ile, Leu, Val]; [13C]-Ile, Leu, Val, 0.8 mM; MgCl2 20 mM

Ex-cond_1: pH: 7.1 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
4D HNCACOnot availablenot availablenot available
4D HNCOCAnot availablenot availablenot available
4D HNCOi-1CAinot availablenot availablenot available
4D 15N,15N-NOESYnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D HN(CO)CACBnot availablenot availablenot available
3D HNCOnot availablenot availablenot available
3D HN(CA)COnot availablenot availablenot available

Software:

NMRView v5.0.4 - data visualization, manual assignments

NMR spectrometers:

  • Varian Inova 800 MHz
  • Varian Inova 600 MHz

Related Database Links:

PDB
DBJ BAE77036 BAG78777 BAI27259 BAI32372 BAI37581
EMBL CAA52639 CAE85241 CAP77433 CAQ33311 CAQ99932
GB AAA69143 AAC76012 AAN44496 AAN82151 AAP18307
REF NP_417450 NP_708789 NP_755578 NP_838497 WP_000005017
SP P37330 P59663 Q8FDN6
AlphaFold P37330 P59663 Q8FDN6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks