BMRB Entry 5467

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5467
MolProbity Validation Chart

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Title:
1H,15N and 13C resonance assignments of the SH3-like domain of the human ADAP protein
Deposition date:
2002-07-19
Original release date:
2004-10-13
Authors:
Gupta, Prateek; Langdon, Grant; Kofler, Michael; Schmidt, Stefanie; Heuer, Katja; Thiemke, Katharina; Freund, Christian
Citation:

Citation: Heuer, Katja; Kofler, Michael; Langdon, Grant; Thiemke, Katharina; Freund, Christian. "Structure of a helically extended SH3 domain of the T cell adapter protein ADAP"  Structure (Cambridge, MA, U. S.) 12, 603-610 (2004).
PubMed: 15062083

Assembly members:

Assembly members:
SH3 like domain of ADAP-binding protein, polymer, 102 residues, 11873.5 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTFT74

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SH3 like domain of ADAP-binding protein: MGSSHHHHHHSSGKKLKKQE KEEKDFRKKFKYDGEIRVLY STKVTTSITSKKWGTRDLQV KPGESLEVIQTTDDTKVLCR NEEGKYGYVLRSYLADNDGE IY

Data sets:
Data typeCount
13C chemical shifts345
15N chemical shifts87
1H chemical shifts560

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SH3 like domain of ADAP protein1

Entities:

Entity 1, SH3 like domain of ADAP protein 102 residues - 11873.5 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLYSLYSLEULYSLYSGLNGLU
3   LYSGLUGLULYSASPPHEARGLYSLYSPHE
4   LYSTYRASPGLYGLUILEARGVALLEUTYR
5   SERTHRLYSVALTHRTHRSERILETHRSER
6   LYSLYSTRPGLYTHRARGASPLEUGLNVAL
7   LYSPROGLYGLUSERLEUGLUVALILEGLN
8   THRTHRASPASPTHRLYSVALLEUCYSARG
9   ASNGLUGLUGLYLYSTYRGLYTYRVALLEU
10   ARGSERTYRLEUALAASPASNASPGLYGLU
11   ILETYR

Samples:

Sample_1: SH3 like domain of ADAP-binding protein, [U-95% 13C; U-90% 15N], 0.5 – 1.8 mM

Ex-cond_1: ionic strength: 0.05 M; pH: 6.3; temperature: 300 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCSample_1not availableEx-cond_1
CBCACONHSample_1not availableEx-cond_1
CBCANHSample_1not availableEx-cond_1
HBHACONHSample_1not availableEx-cond_1
HNCOSample_1not availableEx-cond_1
1H-15N NOESYSample_1not availableEx-cond_1
1H-15N TOCSYSample_1not availableEx-cond_1
1H-13C TOCSYSample_1not availableEx-cond_1
1H-13C NOESYSample_1not availableEx-cond_1
1H-1H TOCSYSample_1not availableEx-cond_1
1H-1H NOESYSample_1not availableEx-cond_1

Software:

SPARKY v3.106 - semi automated peak assignments

XEASY v1.3.13 - peak assignments

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAF83092 BAG59594
GB AAB62226 AAC51300 AAF62400 AAI17450 AAI43646
REF NP_001230022 NP_001456 NP_955367 XP_003274413 XP_003274414
SP O15117
AlphaFold O15117

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks