BMRB Entry 5462

Name: 1H, 15N and 13C Backbone Assignment of MJ1267, an ATP-binding cassette
Published: 2002-11-05

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5462

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N and 13C Backbone Assignment of MJ1267, an ATP-binding cassette

Deposition date:

2002-07-15

Original release date:

2002-11-05

Authors:

Wang, Chunyu; Hunt, John; Rance, Mark; Palmer, Arthur

Citation:

Citation: Wang, Chunyu; Hunt, John; Rance, Mark; Palmer, Arthur. "Letter to the Editor: 1H, 15N and 13C Backbone Assignment of MJ1267, an ATP-binding cassette" J. Biomol. NMR 24, 167-168 (2002).

Assembly members:

Assembly members:
MJ1267, polymer, 253 residues, 28994.7 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: 2190 Superkingdom: Archaea Kingdom: not available Genus/species: Methanococcus jannaschii

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MJ1267: MRDTMEILRTENIVKYFGEF KALDGVSISVNKGDVTLIIG GPNSGKSTLINVITGFLKAD EGRVYFENKDITNKEPAELY HYGIVRTFQTPQPLKEMTVL ENLLIGEINPGESPLNSLFY KKWIPKEEEMVEKAFKILEF LKLSHLYDRKAGELSGGQMK LVEIGRALMTNPKMIVMDEP IAGVAPGLAHDIFNHVLELK AKGITFHRLDIVLNYIDHLY VMFNGQIIAEGRGEEEIKNV LSDPKVVEIYIGE

Data sets:

assigned_chemical_shifts
- chemical_shift_data

Data type	Count
13C chemical shifts	683
15N chemical shifts	223
1H chemical shifts	223

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MJ1267	1

Entities:

Entity 1, MJ1267 253 residues - 28994.7 Da.

1

MET

ARG

ASP

THR

MET

GLU

ILE

LEU

ARG

THR

2

GLU

ASN

ILE

VAL

LYS

TYR

PHE

GLY

GLU

PHE

3

LYS

ALA

LEU

ASP

GLY

VAL

SER

ILE

SER

VAL

4

ASN

LYS

GLY

ASP

VAL

THR

LEU

ILE

GLY

5

GLY

PRO

ASN

SER

GLY

LYS

SER

THR

LEU

ILE

6

ASN

VAL

ILE

THR

GLY

PHE

LEU

LYS

ALA

ASP

7

GLU

GLY

ARG

VAL

TYR

PHE

GLU

ASN

LYS

ASP

8

ILE

THR

ASN

LYS

GLU

PRO

ALA

GLU

LEU

TYR

9

HIS

TYR

GLY

ILE

VAL

ARG

THR

PHE

GLN

THR

10

PRO

GLN

PRO

LEU

LYS

GLU

MET

THR

VAL

LEU

11

GLU

ASN

LEU

ILE

GLY

GLU

ILE

ASN

PRO

12

GLY

GLU

SER

PRO

LEU

ASN

SER

LEU

PHE

TYR

13

LYS

TRP

ILE

PRO

LYS

GLU

MET

14

VAL

GLU

LYS

ALA

PHE

LYS

ILE

LEU

GLU

PHE

15

LEU

LYS

LEU

SER

HIS

LEU

TYR

ASP

ARG

LYS

16

ALA

GLY

GLU

LEU

SER

GLY

GLN

MET

LYS

17

LEU

VAL

GLU

ILE

GLY

ARG

ALA

LEU

MET

THR

18

ASN

PRO

LYS

MET

ILE

VAL

MET

ASP

GLU

PRO

19

ILE

ALA

GLY

VAL

ALA

PRO

GLY

LEU

ALA

HIS

20

ASP

ILE

PHE

ASN

HIS

VAL

LEU

GLU

LEU

LYS

21

ALA

LYS

GLY

ILE

THR

PHE

HIS

ARG

LEU

ASP

22

ILE

VAL

LEU

ASN

TYR

ILE

ASP

HIS

LEU

TYR

23

VAL

MET

PHE

ASN

GLY

GLN

ILE

ALA

GLU

24

GLY

ARG

GLY

GLU

ILE

LYS

ASN

VAL

25

LEU

SER

ASP

PRO

LYS

VAL

GLU

ILE

TYR

26

ILE

GLY

GLU

Samples:

sample_1: MJ1267, [U-13C; U-15N; U-96% 2H], 0.6 mM; sodium phosphate 20 mM

Ex-cond_1: ionic strength: 0.05 M; pH: 7.0 na; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N TROSY	not available	not available	not available
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
HNCACB	not available	not available	not available
HN(CO)CACB	not available	not available	not available

Software:

NMRPipe - data processing

SPARKY - data analysis

NMR spectrometers:

Varian INOVA 600 MHz

GB	AAB99273
REF	NP_248263 WP_010870780
SP	Q58663
AlphaFold	Q58663