BMRB Entry 5458

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5458

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 13C, and 15N assignments of the ribosome recycling factor from Thermus thermophillus
Deposition date:
2002-07-09
Original release date:
2002-12-27
Authors:
Blake, Brain; Ito, Koichi; Nakamura, Yoshikazu; Alam, Steven
Citation:

Citation: Blake, Brain; Ito, Koichi; Nakamura, Yoshikazu; Alam, Steven. "Letter to the Editor: Backbone 1H, 13C, and 15N assignments of the ribosome recycling factor from Thermus thermophillus"  J. Biomol. NMR 24, 81-82 (2002).

Assembly members:

Assembly members:
ribosome recycling factor, polymer, 185 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 274   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Thermus thermophillus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ribosome recycling factor: MTLKELYAETRSHMQKSLEV LEHNLAGLRTGRANPALLLH LKVEYYGAHVPLNQIATVTA PDPRTLVVQSWDQNALKAIE KAIRDSDLGLNPSNKGDALY INIPPLTEERRKDLVRAVRQ YAEEGRVAIRNIRREALDKL KKLAKELHLSEDETKRAEAE IQKITDEFIAKADQLAEKKE QEILG

Data sets:
Data typeCount
1H chemical shifts178
13C chemical shifts541
15N chemical shifts178

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRF1

Entities:

Entity 1, RRF 185 residues - Formula weight is not available

1   METTHRLEULYSGLULEUTYRALAGLUTHR
2   ARGSERHISMETGLNLYSSERLEUGLUVAL
3   LEUGLUHISASNLEUALAGLYLEUARGTHR
4   GLYARGALAASNPROALALEULEULEUHIS
5   LEULYSVALGLUTYRTYRGLYALAHISVAL
6   PROLEUASNGLNILEALATHRVALTHRALA
7   PROASPPROARGTHRLEUVALVALGLNSER
8   TRPASPGLNASNALALEULYSALAILEGLU
9   LYSALAILEARGASPSERASPLEUGLYLEU
10   ASNPROSERASNLYSGLYASPALALEUTYR
11   ILEASNILEPROPROLEUTHRGLUGLUARG
12   ARGLYSASPLEUVALARGALAVALARGGLN
13   TYRALAGLUGLUGLYARGVALALAILEARG
14   ASNILEARGARGGLUALALEUASPLYSLEU
15   LYSLYSLEUALALYSGLULEUHISLEUSER
16   GLUASPGLUTHRLYSARGALAGLUALAGLU
17   ILEGLNLYSILETHRASPGLUPHEILEALA
18   LYSALAASPGLNLEUALAGLULYSLYSGLU
19   GLNGLUILELEUGLY

Samples:

sample_1: ribosome recycling factor, [U-98% 13C; U-98% 15N], 1.05 mM; NH4Cl 50 mM; D2O 10%

sample_2: ribosome recycling factor, [U-98% 15N], 1.05 mM; NH4Cl 50 mM; D2O 10%

sample_3: ribosome recycling factor, [U-98% 2H; U-98% 13C; U-98% 15N], 1.05 mM; NH4Cl 50 mM; D2O 10%

Ex-cond: pH: 6.4 na; temperature: 310 K; ionic strength: 0.10 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableEx-cond
HNCAnot availablenot availableEx-cond
HN(CO)CAnot availablenot availableEx-cond
HNCOnot availablenot availableEx-cond
HN(CA)COnot availablenot availableEx-cond
CBCA(CO)NHnot availablenot availableEx-cond
HNCACBnot availablenot availableEx-cond
(H)C(CO)NHnot availablenot availableEx-cond
1H-15N NOESYnot availablenot availableEx-cond
1H-15N TOCSYnot availablenot availableEx-cond

Software:

SPARKY v1.1 - peak picking, peak assignment

NMR spectrometers:

  • Varian INOVA 750 MHz
  • Varian INOVA 600 MHz
  • Varian UNITY 500 MHz

Related Database Links:

PDB
DBJ BAA76865 BAD70681
GB AAS80854 AEG33280 AFH39081 EIA39843
REF WP_008630695 WP_011172951 WP_014510208 WP_014629718 YP_004481
SP Q72KE0 Q9WX76
AlphaFold Q72KE0 Q9WX76

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks