BMRB Entry 5456
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5456
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Nishida, Noritaka; Miyazawa, Mayumi; Sumikawa, Hiromi; Sakakura, Masayoshi; Shimba, Nobuhisa; Takahashi, Hideo; Terasawa, Hiroaki; Suzuki, Ei-ichiro; Shimada, Ichio. "Letter to the Editor: Backbone 1H, 13C, and 15N resonance assignments of the
von Willebrand factor A3 domain" J. Biomol. NMR 24, 357-358 (2002).
Assembly members:
von Willebrand factor-A3 domain, polymer, 197 residues, 20861 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
von Willebrand factor-A3 domain: GSMDIAPDCSQPLDVILLLD
GSSSFPASYFDEMKSFAKAF
ISKANIGPRLTQVSVLQYGS
ITTIDVPWNVVPEKAHLLSL
VDVMQREGGPSQIGDALGFA
VRYLTSEMHGARPGASKAVV
ILVTDVSVDSVDAAADAARS
NRVTVFPIGIGDRYDAAQLR
ILAGPAGDSNVVKLQRIEDL
PTMVTLGNSFLHKLCSG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 366 |
| 15N chemical shifts | 183 |
| 1H chemical shifts | 183 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | A3 domain | 1 |
Entities:
Entity 1, A3 domain 197 residues - 20861 Da.
| 1 | GLY | SER | MET | ASP | ILE | ALA | PRO | ASP | CYS | SER | ||||
| 2 | GLN | PRO | LEU | ASP | VAL | ILE | LEU | LEU | LEU | ASP | ||||
| 3 | GLY | SER | SER | SER | PHE | PRO | ALA | SER | TYR | PHE | ||||
| 4 | ASP | GLU | MET | LYS | SER | PHE | ALA | LYS | ALA | PHE | ||||
| 5 | ILE | SER | LYS | ALA | ASN | ILE | GLY | PRO | ARG | LEU | ||||
| 6 | THR | GLN | VAL | SER | VAL | LEU | GLN | TYR | GLY | SER | ||||
| 7 | ILE | THR | THR | ILE | ASP | VAL | PRO | TRP | ASN | VAL | ||||
| 8 | VAL | PRO | GLU | LYS | ALA | HIS | LEU | LEU | SER | LEU | ||||
| 9 | VAL | ASP | VAL | MET | GLN | ARG | GLU | GLY | GLY | PRO | ||||
| 10 | SER | GLN | ILE | GLY | ASP | ALA | LEU | GLY | PHE | ALA | ||||
| 11 | VAL | ARG | TYR | LEU | THR | SER | GLU | MET | HIS | GLY | ||||
| 12 | ALA | ARG | PRO | GLY | ALA | SER | LYS | ALA | VAL | VAL | ||||
| 13 | ILE | LEU | VAL | THR | ASP | VAL | SER | VAL | ASP | SER | ||||
| 14 | VAL | ASP | ALA | ALA | ALA | ASP | ALA | ALA | ARG | SER | ||||
| 15 | ASN | ARG | VAL | THR | VAL | PHE | PRO | ILE | GLY | ILE | ||||
| 16 | GLY | ASP | ARG | TYR | ASP | ALA | ALA | GLN | LEU | ARG | ||||
| 17 | ILE | LEU | ALA | GLY | PRO | ALA | GLY | ASP | SER | ASN | ||||
| 18 | VAL | VAL | LYS | LEU | GLN | ARG | ILE | GLU | ASP | LEU | ||||
| 19 | PRO | THR | MET | VAL | THR | LEU | GLY | ASN | SER | PHE | ||||
| 20 | LEU | HIS | LYS | LEU | CYS | SER | GLY |
Samples:
sample_1: von Willebrand factor-A3 domain, [U-98% 13C; U-98% 15N], 0.5 – 0.8 mM
sample_2: von Willebrand factor-A3 domain, [U-98% 2H; U-98% 13C; U-98% 15N], 0.8 mM
Ex_cond_1: pH: 6.0; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D CBCA(CO)NH | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks