BMRB Entry 5455

Name: 1H, 13C and 15N resonance assignments of the human phosphatase PRL-3
Published: 2008-09-18

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5455

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignments of the human phosphatase PRL-3

Deposition date:

2002-07-04

Original release date:

2008-09-18

Authors:

Kozlov, Guennadi; Cheng, Jing; Lievre, Carine; Banville, Denis; Gehring, Kalle; Ekiel, Irena

Citation:

Citation: Kozlov, Guennadi; Cheng, Jing; Lievre, Carine; Banville, Denis; Gehring, Kalle; Ekiel, Irena. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the Human Phosphatase PRL-3" J. Biomol. NMR 24, 169-170 (2002).
PubMed: 12495037

Assembly members:

Assembly members:
PRL-3, polymer, 172 residues, 19383 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PRL-3: GSHMARMNRPAPVEVSYKHM RFLITHNPTNATLSTFIEDL KKYGATTVVRVCEVTYDKTP LEKDGITVVDWPFDDGAPPP GKVVEDWLSLVKAKFCEAPG SCVAVHCVAGLGRAPVLVAL ALIESGMKYEDAIQFIRQKR RGAINSKQLTYLEKYRPKQR LRFKDPHTHKTR

Data sets:

assigned_chemical_shifts
- shift_1

Data type	Count
13C chemical shifts	588
15N chemical shifts	149
1H chemical shifts	911

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PRL-3 subunit 1	1

Entities:

Entity 1, PRL-3 subunit 1 172 residues - 19383 Da.

1

GLY

SER

HIS

MET

ALA

ARG

MET

ASN

ARG

PRO

2

ALA

PRO

VAL

GLU

VAL

SER

TYR

LYS

HIS

MET

3

ARG

PHE

LEU

ILE

THR

HIS

ASN

PRO

THR

ASN

4

ALA

THR

LEU

SER

THR

PHE

ILE

GLU

ASP

LEU

5

LYS

TYR

GLY

ALA

THR

VAL

ARG

6

VAL

CYS

GLU

VAL

THR

TYR

ASP

LYS

THR

PRO

7

LEU

GLU

LYS

ASP

GLY

ILE

THR

VAL

ASP

8

TRP

PRO

PHE

ASP

GLY

ALA

PRO

9

GLY

LYS

VAL

GLU

ASP

TRP

LEU

SER

LEU

10

VAL

LYS

ALA

LYS

PHE

CYS

GLU

ALA

PRO

GLY

11

SER

CYS

VAL

ALA

VAL

HIS

CYS

VAL

ALA

GLY

12

LEU

GLY

ARG

ALA

PRO

VAL

LEU

VAL

ALA

LEU

13

ALA

LEU

ILE

GLU

SER

GLY

MET

LYS

TYR

GLU

14

ASP

ALA

ILE

GLN

PHE

ILE

ARG

GLN

LYS

ARG

15

ARG

GLY

ALA

ILE

ASN

SER

LYS

GLN

LEU

THR

16

TYR

LEU

GLU

LYS

TYR

ARG

PRO

LYS

GLN

ARG

17

LEU

ARG

PHE

LYS

ASP

PRO

HIS

THR

HIS

LYS

18

THR

ARG

Samples:

sample_1: PRL-3, [U-15N; U-13C], 2.5 – 3.0 mM

cond1: ionic strength: 0.15 M; pH: 6.8; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCACB	not available	not available	not available
CBCACONH	not available	not available	not available
HNCO	not available	not available	not available
HNCA	not available	not available	not available
1H-13C HSQC	not available	not available	not available
3D HCCH-TOCSY	not available	not available	not available
1H-15N TOCSY	not available	not available	not available
1H-15N NOESY	not available	not available	not available

Software:

XEASY - assignments

GIFA - processing

NMR spectrometers:

Bruker DRX 500 MHz

BMRB	19395
PDB	1R6H 1V3A 2MBC
DBJ	BAJ20896
GB	AAC29314 EHH25482 EHH64453
REF	NP_001180623 NP_116000 XP_002819531 XP_003819536 XP_003903255
SP	O75365
AlphaFold	O75365