BMRB Entry 5446

Title:
pKa determinations for P2-Val,P3'-Ala OMTKY3
Deposition date:
2002-07-04
Original release date:
2003-03-20
Authors:
Song, Jikui; Laskowski, Michael; Qasim, Mohammad; Markley, John
Citation:

Citation: Song, Jikui; Laskowski, Michael; Qasim, Mohammad; Markley, John. "NMR Determination of pKa Values for Asp, Glu, His, and Lys Mutants at Each Variable Contiguous Enzyme-inhibitor Contact Position of the Turkey Ovomucoid Third Domain"  Biochemistry 42, 2847-2856 (2003).
PubMed: 12627950

Assembly members:

Assembly members:
Turkey Ovomucoid Third Domain, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Turkey   Taxonomy ID: 9103   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Meleagris gallopavo

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Turkey Ovomucoid Third Domain: LAAVSVDCSEYPKPACVLEY APLCGSDNKTYGNKCNFCNA VVESNGTLTLSHFGKC

Data sets:
Data typeCount
pH NMR parameter values16
pKa values1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1P2-Val,P3'-Ala OMTKY31

Entities:

Entity 1, P2-Val,P3'-Ala OMTKY3 56 residues - Formula weight is not available

1   LEUALAALAVALSERVALASPCYSSERGLU
2   TYRPROLYSPROALACYSVALLEUGLUTYR
3   ALAPROLEUCYSGLYSERASPASNLYSTHR
4   TYRGLYASNLYSCYSASNPHECYSASNALA
5   VALVALGLUSERASNGLYTHRLEUTHRLEU
6   SERHISPHEGLYLYSCYS

Samples:

sample_1: Turkey Ovomucoid Third Domain 2 mM; KCl 100 mM; DSS 0.4 mM

conditions_set_1: ionic strength: 100 mM; pH: .; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
TOCSYsample_1not availableconditions_set_1

Software:

No software information available

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 5416