BMRB Entry 5408
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5408
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Citation: Fukushima, Kay; Kikuchi, Jun; Koshiba, Seizo; Kigawa, Takanori; Kuroda, Yutaka; Yokoyama, Shigeyuki. "Solution Structure of the DFF-C Domain of DFF45/ICAD. A Structural
Basis for the Regulation of Apoptotic DNA Fragmentation" J. Mol. Biol. 321, 317-327 (2002).
PubMed: 12144788
Assembly members:
DNA flagmentation factor 45kDa, polymer, 107 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15-b
Entity Sequences (FASTA):
DNA flagmentation factor 45kDa: TGISRETSSDVALASHILTA
LREKQAPELSLSSQDLELVT
KEDPKALAVALNWDIKKTET
VQEACERELALRLQQTQSLH
SLRSISASKASPPGDLQNPK
RARQDPT
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 163 |
| 13C chemical shifts | 157 |
| 15N chemical shifts | 80 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DFF45 C-terminal domain | 1 |
Entities:
Entity 1, DFF45 C-terminal domain 107 residues - Formula weight is not available
| 1 | THR | GLY | ILE | SER | ARG | GLU | THR | SER | SER | ASP | ||||
| 2 | VAL | ALA | LEU | ALA | SER | HIS | ILE | LEU | THR | ALA | ||||
| 3 | LEU | ARG | GLU | LYS | GLN | ALA | PRO | GLU | LEU | SER | ||||
| 4 | LEU | SER | SER | GLN | ASP | LEU | GLU | LEU | VAL | THR | ||||
| 5 | LYS | GLU | ASP | PRO | LYS | ALA | LEU | ALA | VAL | ALA | ||||
| 6 | LEU | ASN | TRP | ASP | ILE | LYS | LYS | THR | GLU | THR | ||||
| 7 | VAL | GLN | GLU | ALA | CYS | GLU | ARG | GLU | LEU | ALA | ||||
| 8 | LEU | ARG | LEU | GLN | GLN | THR | GLN | SER | LEU | HIS | ||||
| 9 | SER | LEU | ARG | SER | ILE | SER | ALA | SER | LYS | ALA | ||||
| 10 | SER | PRO | PRO | GLY | ASP | LEU | GLN | ASN | PRO | LYS | ||||
| 11 | ARG | ALA | ARG | GLN | ASP | PRO | THR |
Samples:
sample1: DNA flagmentation factor 45kDa0.5 – 3.0 mM
sample_conditions: pH: 6.5; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 15N HSQC | not available | not available | not available |
| 15N NOESY-HSQC | not available | not available | not available |
| 15N TOCSY-HSQC | not available | not available | not available |
| 13C HSQC | not available | not available | not available |
| 13C NOESY-HSQC | not available | not available | not available |
| HCCH-TOCSY | not available | not available | not available |
| HCCH-COSY | not available | not available | not available |
| HNCO | not available | not available | not available |
| HN(CO)CA | not available | not available | not available |
| HNCA | not available | not available | not available |
| CBCA(CO)NH | not available | not available | not available |
| CBCANH | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Bruker DRX 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAD96266 BAG36122 BAJ20745 |
| GB | AAC51249 AAH00037 AAH07112 AAH07721 AAP35626 |
| REF | NP_004392 XP_004024685 |
| SP | O00273 |
| AlphaFold | O00273 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
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