BMRB Entry 5406

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5406

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for Human Cytochrome c
Deposition date:
2002-06-27
Original release date:
2003-07-30
Authors:
Jeng, Wen-Yih; Chuang, Woei-Jer
Citation:

Citation: Jeng, Wen-Yih; Chen, Chiu-Yueh; Chang, Hsien-Chang; Chuang, Woei-Jer. "Expression and Characterization of Recombinant Human Cytochrome c in E. coli"  J. Bioenerg. Biomembr. 34, 423-431 (2002).
PubMed: 12678434

Assembly members:

Assembly members:
cytochrome c, polymer, 104 residues, 12234 Da.
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pPET-21b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cytochrome c: GDVEKGKKIFIMKCSQCHTV EKGGKHKTGPNLHGLFGRKT GQAPGYSYTAANKNKGIIWG EDTLMEYLENPKKYIPGTKM IFVGIKKKEERADLIAYLKK ATNE

Data sets:
Data typeCount
1H chemical shifts756
13C chemical shifts219
15N chemical shifts103

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cytochrome c peptide1
2heme c2

Entities:

Entity 1, cytochrome c peptide 104 residues - 12234 Da.

1   GLYASPVALGLULYSGLYLYSLYSILEPHE
2   ILEMETLYSCYSSERGLNCYSHISTHRVAL
3   GLULYSGLYGLYLYSHISLYSTHRGLYPRO
4   ASNLEUHISGLYLEUPHEGLYARGLYSTHR
5   GLYGLNALAPROGLYTYRSERTYRTHRALA
6   ALAASNLYSASNLYSGLYILEILETRPGLY
7   GLUASPTHRLEUMETGLUTYRLEUGLUASN
8   PROLYSLYSTYRILEPROGLYTHRLYSMET
9   ILEPHEVALGLYILELYSLYSLYSGLUGLU
10   ARGALAASPLEUILEALATYRLEULYSLYS
11   ALATHRASNGLU

Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Samples:

sample_1: cytochrome c 2 mM

sample_2: cytochrome c, [U-15N], 2 mM

sample_3: cytochrome c, [U-13C; U-15N], 2 mM

sample_4: cytochrome c, selective 15N-G, 0.5 mM

cond._set_1: pH: 6.5; temperature: 300 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablecond._set_1
1H-1H TOCSYnot availablenot availablecond._set_1
1H-15N HSQCnot availablenot availablecond._set_1
1H-1H-15N NOESYnot availablenot availablecond._set_1
1H-1H-15N TOCSYnot availablenot availablecond._set_1
13C-1H-1H NOESYnot availablenot availablecond._set_1
1H-13C-1H HCCH-TOCSYnot availablenot availablecond._set_1
1H-13C-1H HCCH-COSYnot availablenot availablecond._set_1
1H-13C-1H HCCH-NOESYnot availablenot availablecond._set_1
HNCACBnot availablenot availablecond._set_1
CBCA(CO)NHnot availablenot availablecond._set_1
HBHA(CBCA)NHnot availablenot availablecond._set_1
HBHA(CBCACO)NHnot availablenot availablecond._set_1

Software:

xwinnmr v2.6 -

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

SWISS-PROT Q5RFH4 Q52V08 P99999 P99998 P00002
REF XP_001140708 XP_001095458 XP_001095344 NP_061820 NP_001065289
PRF 630485A
GenBank AAH09578 AAH08477 AAH08475 AAH05299 AAA35732
EMBL CAL38301 CAL38091 CAH89483 CAG46972 CAD28485
DBJ BAG34778
PDB

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks