Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR54037
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Manigrasso, Jacopo; Mestdach, Emeline; Quaia, Riccardo; Dankova, Daniela; Bood, Mattias; Lamurell, Malin; Frolov, Andrey; D. Disney, Mathew; Hogner, Anders; Geschwindner, Stefan; Chi, Celestine; La Sala, Giuseppina. "Conformational dynamics at the pre-miR-377 Dicer site governs selective small-molecule recognition
" Nature ., .-..
Assembly members:
entity_1, polymer, 27 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: obtained from a vendor
Entity Sequences (FASTA):
entity_1: GGAAUUCGCUUUAUUUAUGU
UGAAUCC
| Data type | Count |
| 13C chemical shifts | 62 |
| 15N chemical shifts | 11 |
| 1H chemical shifts | 162 |
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | mir377 | 1 |
Entity 1, mir377 27 residues - Formula weight is not available
| 1 | G | G | A | A | U | U | C | G | C | U | ||||
| 2 | U | U | A | U | U | U | A | U | G | U | ||||
| 3 | U | G | A | A | U | C | C |
sample_1: mir377, [U-100% 15N], 1100 uM; mir377, [U-100% 13C], 1100 uM; DSS 10 uM
sample_conditions_1: ionic strength: 150 mM; pH: 7.4; pressure: 1 atm; temperature: 290 K
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
CcpNMR - chemical shift assignment, data analysis