BMRB Entry 5399
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5399
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Mackereth, Cameron; Scharpf, Manuela; Gentile, Lisa; McIntosh, Lawrence. "Letters to the Editor: New NMR assignments chemical shift and secondary structure
conservation of the PNT/SAM domains from the Ets family of transcription factors" J. Biomol. NMR 24, 71-72 (2002).
Assembly members:
Erg Pointed Domain, polymer, 97 residues, 11381 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a
Entity Sequences (FASTA):
Erg Pointed Domain: GSHMEEKHMPPPNMTTNERR
VIVPADPTLWSTDHVRQWLE
WAVKEYGLPDVNILLFQNID
GKELCKMTKDDFQRLTPSYN
ADILLSHLHYLRETPLP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 671 |
| 13C chemical shifts | 432 |
| 15N chemical shifts | 112 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Erg Pointed domain | 1 |
Entities:
Entity 1, Erg Pointed domain 97 residues - 11381 Da.
| 1 | GLY | SER | HIS | MET | GLU | GLU | LYS | HIS | MET | PRO | ||||
| 2 | PRO | PRO | ASN | MET | THR | THR | ASN | GLU | ARG | ARG | ||||
| 3 | VAL | ILE | VAL | PRO | ALA | ASP | PRO | THR | LEU | TRP | ||||
| 4 | SER | THR | ASP | HIS | VAL | ARG | GLN | TRP | LEU | GLU | ||||
| 5 | TRP | ALA | VAL | LYS | GLU | TYR | GLY | LEU | PRO | ASP | ||||
| 6 | VAL | ASN | ILE | LEU | LEU | PHE | GLN | ASN | ILE | ASP | ||||
| 7 | GLY | LYS | GLU | LEU | CYS | LYS | MET | THR | LYS | ASP | ||||
| 8 | ASP | PHE | GLN | ARG | LEU | THR | PRO | SER | TYR | ASN | ||||
| 9 | ALA | ASP | ILE | LEU | LEU | SER | HIS | LEU | HIS | TYR | ||||
| 10 | LEU | ARG | GLU | THR | PRO | LEU | PRO |
Samples:
15N_Erg_PNT: Erg Pointed Domain, [U-99% 15N], 0.5 – 2.5 mM
13C-15N_Erg_PNT: Erg Pointed Domain, [U-99% 13C; U-99% 15N], 1.5 mM
10_13C-15N_Erg_PNT: Erg Pointed Domain, [U-10% 13C; U-99% 15N], 1.5 mM
sample_conditions: pH: 7.0; temperature: 303 K; ionic strength: 0.05 M
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N HSQC | not available | not available | not available |
| 1H-13C HSQC | not available | not available | not available |
| HNCACB | not available | not available | not available |
| HBCBCACONH | not available | not available | not available |
| HNCO | not available | not available | not available |
| HACAN | not available | not available | not available |
| H(CCO)NH-TOCSY | not available | not available | not available |
| C(CO)NH-TOCSY | not available | not available | not available |
| HHCH-TOCSY | not available | not available | not available |
Software:
FELIX v2000 - processing spectra
Sparky v3 - chemical shift assignment
NMR spectrometers:
- Varian INOVA 600 MHz
- Varian Unity 500 MHz
Related Database Links:
| PDB | |
| DBJ | BAG60145 BAG62127 BAG62837 BAG64546 BAH24130 |
| EMBL | CAA54404 CAL37450 |
| GB | AAA35811 AAA52398 AAH40168 AAI48055 AAP41719 |
| REF | NP_001095653 NP_001129626 NP_001129627 NP_001230357 NP_001230358 |
| SP | P11308 Q90837 |
| TPG | DAA32951 |
| AlphaFold | P11308 Q90837 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks