BMRB Entry 5397

Name: 1H, 13C and 15N resonance assignment of an affibody-target complex
Published: 2002-12-23

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5397

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignment of an affibody-target complex

Deposition date:

2002-06-14

Original release date:

2002-12-23

Authors:

Lendel, Christofer; Wahlberg, Elisabet; Berglund, Helena; Eklund, Malin; Nygren, Per-Ake; Hard, Torleif

Citation:

Citation: Lendel, Christofer; Wahlberg, Elisabet; Berglund, Helena; Eklund, Malin; Nygren, Per-Ake; Hard, Torleif. "Letter to the Editor: 1H, 13C and 15N resonance assignments of an affibody-target complex " J. Biomol. NMR 24, 271-272 (2002).

Assembly members:

Assembly members:
Z-domain, polymer, 58 residues, Formula weight is not available
ZSPA-1, polymer, 58 residues, Formula weight is not available

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Z-domain: VDNKFNKEQQNAFYEILHLP NLNEEQRNAFIQSLKDDPSQ SANLLAEAKKLNDAQAPK
ZSPA-1: VDNKFNKELSVAGREIVTLP NLNDPQKKAFIFSLWDDPSQ SANLLAEAKKLNDAQAPK

Data sets:

assigned_chemical_shifts
- shift_set_Z-domain
- shift_set_ZSPA-1

Data type	Count
1H chemical shifts	852
13C chemical shifts	500
15N chemical shifts	134

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Z-domain	1
2	ZSPA-1 affibody	2

Entities:

Entity 1, Z-domain 58 residues - Formula weight is not available

1

VAL

ASP

ASN

LYS

PHE

ASN

LYS

GLU

GLN

2

ASN

ALA

PHE

TYR

GLU

ILE

LEU

HIS

LEU

PRO

3

ASN

LEU

ASN

GLU

GLN

ARG

ASN

ALA

PHE

4

ILE

GLN

SER

LEU

LYS

ASP

PRO

SER

GLN

5

SER

ALA

ASN

LEU

ALA

GLU

ALA

LYS

6

LEU

ASN

ASP

ALA

GLN

ALA

PRO

LYS

Entity 2, ZSPA-1 affibody 58 residues - Formula weight is not available

1

VAL

ASP

ASN

LYS

PHE

ASN

LYS

GLU

LEU

SER

2

VAL

ALA

GLY

ARG

GLU

ILE

VAL

THR

LEU

PRO

3

ASN

LEU

ASN

ASP

PRO

GLN

LYS

ALA

PHE

4

ILE

PHE

SER

LEU

TRP

ASP

PRO

SER

GLN

5

SER

ALA

ASN

LEU

ALA

GLU

ALA

LYS

6

LEU

ASN

ASP

ALA

GLN

ALA

PRO

LYS

Samples:

Sample_1: Z-domain, [U-13C; U-15N], 1.5 – 2.0 mM; ZSPA-11.65 – 2.5 mM; potassium phosphate 20 mM; sodium azide 0.01%; D2O10 – 100%

Sample_2: ZSPA-1, [U-13C; U-15N], 1.5 – 2.0 mM; Z-domain1.65 – 2.5 mM; potassium phosphate 20 mM; sodium azide 0.01%; D2O10 – 100%

Conditions: pH*: 5.6; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
1H-13C HSQC	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
CBCANH	not available	not available	not available
HNCO	not available	not available	not available
HN(CA)CO	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
HCCH-COSY	not available	not available	not available
15N-TOCSY-HSQC	not available	not available	not available
15N-NOESY-HSQC	not available	not available	not available

Software:

XWIN-NMR - data processing

NMRpipe - data processing

Ansig for Windows - analysis and assignment

NMR spectrometers:

Bruker AVANCE 500 MHz
Bruker AVANCE 600 MHz
Varian INOVA 800 MHz