BMRB Entry 53962
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53962
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NMR-STAR v3 text file.
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Citation: Couture, Heather; Feng, Wei; Wang, Xu. "Conformation Dynamics as an Explanation for the Ligand Promiscuity of aMI-domain
" JBC ., .-..
Assembly members:
entity_1, polymer, 186 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pHUE
- heteronucl_T2_relaxation
| Data type | Count |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | aLID | 1 |
Entities:
Entity 1, aLID 186 residues - Formula weight is not available
Numbering starts at 127.
| 1 | LYS | GLY | ASN | VAL | ASP | LEU | VAL | PHE | LEU | PHE | ||||
| 2 | ASP | GLY | SER | MET | SER | LEU | GLN | PRO | ASP | GLU | ||||
| 3 | PHE | GLN | LYS | ILE | LEU | ASP | PHE | MET | LYS | ASP | ||||
| 4 | VAL | MET | LYS | LYS | LEU | SER | ASN | THR | SER | TYR | ||||
| 5 | GLN | PHE | ALA | ALA | VAL | GLN | PHE | SER | THR | SER | ||||
| 6 | TYR | LYS | THR | GLU | PHE | ASP | PHE | SER | ASP | TYR | ||||
| 7 | VAL | LYS | TRP | LYS | ASP | PRO | ASP | ALA | LEU | LEU | ||||
| 8 | LYS | HIS | VAL | LYS | HIS | MET | LEU | LEU | LEU | THR | ||||
| 9 | ASN | THR | PHE | GLY | ALA | ILE | ASN | TYR | VAL | ALA | ||||
| 10 | THR | GLU | VAL | PHE | ARG | GLU | GLU | LEU | GLY | ALA | ||||
| 11 | ARG | PRO | ASP | ALA | THR | LYS | VAL | LEU | ILE | ILE | ||||
| 12 | ILE | THR | ASP | GLY | GLU | ALA | THR | ASP | SER | GLY | ||||
| 13 | ASN | ILE | ASP | ALA | ALA | LYS | ASP | ILE | ILE | ARG | ||||
| 14 | TYR | ILE | ILE | GLY | ILE | GLY | LYS | HIS | PHE | GLN | ||||
| 15 | THR | LYS | GLU | SER | GLN | GLU | THR | LEU | HIS | LYS | ||||
| 16 | PHE | ALA | SER | LYS | PRO | ALA | SER | GLU | PHE | VAL | ||||
| 17 | LYS | ILE | LEU | ASP | THR | PHE | GLU | LYS | LEU | LYS | ||||
| 18 | ASP | LEU | PHE | THR | GLU | LEU | GLN | LYS | LYS | ILE | ||||
| 19 | TYR | VAL | ILE | GLU | GLY | THR |
Samples:
sample_1: aLID, [U-100% 15N], 0.85 ± 0.05 mM; HEPES 20 ± 1 mM; sodium chloride 100 ± 5 mM; D2O 10 ± 1 v/v
sample_conditions_1: ionic strength: 0.12 M; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| T2/R2 relaxation | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe - processing
TOPSPIN - collection
NMRView - data analysis
Relax v4.0.3 - data analysis
NMR spectrometers:
- Varian INVOVA 800 MHz