BMRB Entry 53960

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53960

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Title:
15N Single Quantum Relaxation Dispersion of Apo Alpha M I-domain
Deposition date:
2026-08-05
Original release date:
2026-09-08
Authors:
Couture, Heather; Wang, Xu
Citation:

Citation: Couture, Heather; Feng, Wei; Wang, Xu. "Conformation Dynamics as an Explanation for the Ligand Promiscuity of aMI-domain "  JBC ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 194 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pHUE

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: EDSDIAFLIDGSGSIIPHDF RRMKEFVSTVMEQLKKSKTL FSLMQYSEEFRIHFTFKEFQ NNPNPRSLVKPITQLLGRTH TATGIRKVVRELFNITNGAR KNAFKILVVITDGEKFGDPL GYEDVIPEADREGVIRYVIG VGDAFRSEKSRQELNTIASK PPRDHVFQVNNFEALKTIQN QLREKIFAIEGTQT

Data sets:
Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1aMID1

Entities:

Entity 1, aMID 194 residues - Formula weight is not available

Numbering starts at 138.

1   GLUASPSERASPILEALAPHELEUILEASP
2   GLYSERGLYSERILEILEPROHISASPPHE
3   ARGARGMETLYSGLUPHEVALSERTHRVAL
4   METGLUGLNLEULYSLYSSERLYSTHRLEU
5   PHESERLEUMETGLNTYRSERGLUGLUPHE
6   ARGILEHISPHETHRPHELYSGLUPHEGLN
7   ASNASNPROASNPROARGSERLEUVALLYS
8   PROILETHRGLNLEULEUGLYARGTHRHIS
9   THRALATHRGLYILEARGLYSVALVALARG
10   GLULEUPHEASNILETHRASNGLYALAARG
11   LYSASNALAPHELYSILELEUVALVALILE
12   THRASPGLYGLULYSPHEGLYASPPROLEU
13   GLYTYRGLUASPVALILEPROGLUALAASP
14   ARGGLUGLYVALILEARGTYRVALILEGLY
15   VALGLYASPALAPHEARGSERGLULYSSER
16   ARGGLNGLULEUASNTHRILEALASERLYS
17   PROPROARGASPHISVALPHEGLNVALASN
18   ASNPHEGLUALALEULYSTHRILEGLNASN
19   GLNLEUARGGLULYSILEPHEALAILEGLU
20   GLYTHRGLNTHR

Samples:

sample_1: aMID, [U-100% 15N], 0.85 ± 0.05 mM; HEPES 20 ± 1 mM; sodium chloride 100 ± 5 mM; D2O 10 ± 1 v/v

sample_conditions_1: ionic strength: 0.12 M; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
T2/R2 relaxationsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

TOPSPIN - collection

NMRView - data analysis

Relax v4.0.3 - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Varian INVOVA 800 MHz

Related Database Links:

UNP p11215
AlphaFold P11215