BMRB Entry 5395

Name: Chemical Shift Assignments for the Unmodified Branch Site Helix
Published: 2003-02-20

Click here to enlarge.

PDB ID: 1lmv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5395
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Chemical Shift Assignments for the Unmodified Branch Site Helix

Deposition date:

2002-06-12

Original release date:

2003-02-20

Authors:

Newby, Meredith; Greenbaum, Nancy

Citation:

Citation: Newby, Meredith; Greenbaum, Nancy. "Sculpting of the Spliceosomal Branch Site Recognition Motif by a Conserved Pseudouridine" Nat. Struct. Biol. 9, 958-965 (2002).
PubMed: 12426583

Assembly members:

Assembly members:
U2 snRNA - intron duplex, polymer, 9 residues, Formula weight is not available
Intron branch site region, polymer, 10 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U2 snRNA - intron duplex: GGUGUAGUA
Intron branch site region: UACUAACACC

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1
- Chemical_shift_assignments_2

Data type	Count
1H chemical shifts	93

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Unmodified U2 snRNA strand	1
2	Intron strand	2

Entities:

Entity 1, Unmodified U2 snRNA strand 9 residues - Formula weight is not available

1

G

U

G

U

A

G

U

A

Entity 2, Intron strand 10 residues - Formula weight is not available

1

U

A

C

U

A

C

A

C

Samples:

sample_1: U2 snRNA - intron duplex 1.0 mM; Intron branch site region 1.0 mM; sodium phosphate 10 mM; D2O 10%; H2O 90%; EDTA 0.1 mM

sample_2: U2 snRNA - intron duplex 1.0 mM; Intron branch site region 1.0 mM; sodium phosphate 10 mM; D2O 100%; EDTA 0.1 mM

sample_cond_1: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 278 K

sample_cond_2: ionic strength: 50 mM; pH: 6.4; pressure: 1 atm; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
2D TOCSY	not available	not available	not available
DQF-COSY	not available	not available	not available

Software:

VNMR v5.3 - collection

SPARKY v3.69 - data analysis

X-PLOR v3.851 - refinement, structure solution

NMR spectrometers:

Varian UNITYplus 720 MHz