Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53939

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and aliphatic carbon resonance assignments of the human synaptopodin actin-binding region

Deposition date:

2026-07-27

Original release date:

2026-08-17

Authors:

Pawlyta, Max; Mulder, Frans

Citation:

Citation: Pawlyta, Max; Mulder, Frans. "Resonance assignment of the actin-binding region of synaptopodin. " Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: SMFTFVEKPKVTPNPDLLDL VQTADEKRRQRDQGEVGVEE EPFALGAEASNFQQEPAPRD RASPAAAEEVVPEWASCLKS PRIQAKPKPKPNQNLSEASG KGAELYARRQSRMEKYVIES SSHTPELARCPSPTMSLPSS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	481
15N chemical shifts	138
1H chemical shifts	122

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SynpoABS	1

Entities:

Entity 1, SynpoABS 140 residues - Formula weight is not available

Residues 379-518 of full length protein

1	SER	MET	PHE	THR	PHE	VAL	GLU	LYS	PRO	LYS
2	VAL	THR	PRO	ASN	PRO	ASP	LEU	LEU	ASP	LEU
3	VAL	GLN	THR	ALA	ASP	GLU	LYS	ARG	ARG	GLN
4	ARG	ASP	GLN	GLY	GLU	VAL	GLY	VAL	GLU	GLU
5	GLU	PRO	PHE	ALA	LEU	GLY	ALA	GLU	ALA	SER
6	ASN	PHE	GLN	GLN	GLU	PRO	ALA	PRO	ARG	ASP
7	ARG	ALA	SER	PRO	ALA	ALA	ALA	GLU	GLU	VAL
8	VAL	PRO	GLU	TRP	ALA	SER	CYS	LEU	LYS	SER
9	PRO	ARG	ILE	GLN	ALA	LYS	PRO	LYS	PRO	LYS
10	PRO	ASN	GLN	ASN	LEU	SER	GLU	ALA	SER	GLY
11	LYS	GLY	ALA	GLU	LEU	TYR	ALA	ARG	ARG	GLN
12	SER	ARG	MET	GLU	LYS	TYR	VAL	ILE	GLU	SER
13	SER	SER	HIS	THR	PRO	GLU	LEU	ALA	ARG	CYS
14	PRO	SER	PRO	THR	MET	SER	LEU	PRO	SER	SER

Samples:

sample_1: SynpoABS, [U-100% 13C; U-100% 15N], 350 uM; D2O, [U-2H], 5 % v/v; DSS 100 uM; sodium chloride 50 mM; sodium phosphate 20 mM; TCEP 2 mM

sample_conditions_1: ionic strength: 77 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D (HN)CO(CO)NH	sample_1	isotropic	sample_conditions_1
3D (H)CC(CO)NH	sample_1	isotropic	sample_conditions_1
2D H(CA)CON	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v4.4.0 - collection, processing

NMRPipe v2023.129.13.28 - processing

CcpNMR v3.3.4.1 - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III 700 MHz

BMRB Entry 53939