BMRB Entry 53908

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53908

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Title:
TDP-43 CTD Oxidized
Deposition date:
2026-07-07
Original release date:
2026-07-17
Authors:
Puterbaugh, Ryan; Viswanathan, Renjith; Fawzi, Nicolas
Citation:

Citation: Puterbaugh, Ryan; Viswanathan, Renjith; Fawzi, Nicolas. "Methionine oxidation alters both helical assembly and disordered contacts in human TDP-43 C-terminal domain phase separation "  PNAS ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 148 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJ411

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NRQLERSGRFGGNPGGFGNQ GGFGNSRGGGAGLGNNQGSN XGGGXNFGAFSINPAXXAAA QAALQSSWGXXGXLASQQNQ SGPSGNNQNQGNXQREPNQA FGSGNNSYSGSNSGAAIGWG SASNAGSGSGFNGGFGSSXD SKSSGWGX

Data sets:
Data typeCount
13C chemical shifts145
15N chemical shifts142
1H chemical shifts142
T2 relaxation values121

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TDP-43 CTD Oxidized1

Entities:

Entity 1, TDP-43 CTD Oxidized 148 residues - Formula weight is not available

1   ASNARGGLNLEUGLUARGSERGLYARGPHE
2   GLYGLYASNPROGLYGLYPHEGLYASNGLN
3   GLYGLYPHEGLYASNSERARGGLYGLYGLY
4   ALAGLYLEUGLYASNASNGLNGLYSERASN
5   SMEGLYGLYGLYSMEASNPHEGLYALAPHE
6   SERILEASNPROALASMESMEALAALAALA
7   GLNALAALALEUGLNSERSERTRPGLYSME
8   SMEGLYSMELEUALASERGLNGLNASNGLN
9   SERGLYPROSERGLYASNASNGLNASNGLN
10   GLYASNSMEGLNARGGLUPROASNGLNALA
11   PHEGLYSERGLYASNASNSERTYRSERGLY
12   SERASNSERGLYALAALAILEGLYTRPGLY
13   SERALASERASNALAGLYSERGLYSERGLY
14   PHEASNGLYGLYPHEGLYSERSERSMEASP
15   SERLYSSERSERGLYTRPGLYSME

Samples:

sample_1: TDP-43 CTD Oxidized, [U-100% 13C; U-100% 15N], 55 uM

sample_conditions_1: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
T2/R2 relaxationsample_1isotropicsample_conditions_1

Software:

NMRFAM-SPARKY - chemical shift assignment

NMRPipe - data analysis

NMR spectrometers:

  • Bruker Avance 850 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks