BMRB Entry 53884

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53884

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Title:
TLR1 transmembrane domain with juxtamembrane hydrophobic cytoplasmic region in bicelles DMPC/DHPC
Deposition date:
2026-06-25
Original release date:
2026-10-13
Authors:
Kornilov, Feudor; Bocharov, Eduard; Goncharuk, Sergey; Mineev, Konstantin
Citation:

Citation: Kornilov, Feudor; Shabalkina, Alexandra; Goncharuk, Marina; Bocharov, Eduard; Goncharuk, Sergey; Mineev, Konstantin. "Transmembrane domains of TLR1 and TLR2 tend to specific heterotypic interaction "  Biochem. Biophys. Res. Commun. 830, 154252-154252 (2026).
PubMed: 42424932

Assembly members:

Assembly members:
entity_1, polymer, 55 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: cell free synthesis   Host organism: Escherichia coli   Vector: pGEMEX-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MKDFHMSELSCNITLLIVTI VATMLVLAVTVTSLCSYLDL PWYLRMVCQWTQTRR

Data sets:
Data typeCount
13C chemical shifts175
15N chemical shifts41
1H chemical shifts320

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Toll-like receptor 11

Entities:

Entity 1, Toll-like receptor 1 55 residues - Formula weight is not available

1   METLYSASPPHEHISMETSERGLULEUSER
2   CYSASNILETHRLEULEUILEVALTHRILE
3   VALALATHRMETLEUVALLEUALAVALTHR
4   VALTHRSERLEUCYSSERTYRLEUASPLEU
5   PROTRPTYRLEUARGMETVALCYSGLNTRP
6   THRGLNTHRARGARG

Samples:

sample_1: Toll-like receptor 1, [U-13C; U-15N], 380 uM; DMPC 9 mg; DHPC 17 mg; sodium phosphate 25 mM; TCEP 4 mM

sample_conditions_1: ionic strength: 25 mM; pH: 7.0; pressure: 1 atm; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
1D 1Hsample_1isotropicsample_conditions_1
2D 1H-15N TROSYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

CARA - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Related Database Links:

UNP Q15399
AlphaFold Q15399

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks