BMRB Entry 53815

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53815

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
HSPB8_ACD_6M_UREA
Deposition date:
2026-05-22
Original release date:
2026-06-29
Authors:
Amin, Zainab; Chugh, Jeetender
Citation:

Citation: Amin, Zainab; Chugh, Jeetender. "Backbone resonance assignment of Human Heat Shock Protein B8 (HSPB8) across various denaturant conditions: A key to understanding its folding and function "  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GGSEFTATARFGVPAEGRTP PPFPGEPWKVCVNVHSFKPE ELMVKTKDGYVEVSGKHEEK QQEGGIVSKNFTKKIQLPAE VDPVTVFASLSPEGLLIIEA PQVPPYSTFGESSFNNE

Data sets:
Data typeCount
13C chemical shifts321
15N chemical shifts102
1H chemical shifts596

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HSPB81

Entities:

Entity 1, HSPB8 117 residues - Formula weight is not available

1   GLYGLYSERGLUPHETHRALATHRALAARG
2   PHEGLYVALPROALAGLUGLYARGTHRPRO
3   PROPROPHEPROGLYGLUPROTRPLYSVAL
4   CYSVALASNVALHISSERPHELYSPROGLU
5   GLULEUMETVALLYSTHRLYSASPGLYTYR
6   VALGLUVALSERGLYLYSHISGLUGLULYS
7   GLNGLNGLUGLYGLYILEVALSERLYSASN
8   PHETHRLYSLYSILEGLNLEUPROALAGLU
9   VALASPPROVALTHRVALPHEALASERLEU
10   SERPROGLUGLYLEULEUILEILEGLUALA
11   PROGLNVALPROPROTYRSERTHRPHEGLY
12   GLUSERSERPHEASNASNGLU

Samples:

sample_1: HSPB8, [U-100% 13C; U-100% 15N], 500 uM; D2O, [U-2H], 10%; H2O 90%; DSS 5 mM; DTT 1 mM; urea 6 M; sodium chloride 100 mM; EDTA 1 mM; sodium phosphate 10 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
HNNsample_1isotropicsample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

  • Bruker AVANCE III 600 MHz

Related Database Links:

UNP Q9UJY1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks