Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53787

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Backbone 1H, 13C, and 15N Chemical Shift Assignments for Nupr1
Published: 2026-05-26

Title:

Backbone 1H, 13C, and 15N Chemical Shift Assignments for Nupr1

Deposition date:

2026-05-18

Original release date:

2026-05-26

Authors:

Kim, Minsu; Choi, Joonhyeok; Cheong, Hae-Kap; Ryu, Kyoung-Seok; Park, Jin-Wan; Ha, Jung-Hye; Im, Jong-Soo; Son, Woo Sung; Wu, Sangwook; Lee, Donghan

Citation:

Citation: Kim, Minsu; Choi, Joonhyeok; Cheong, Hae-Kap; Ryu, Kyoung-Seok; Park, Jin-Wan; Ha, Jung-Hye; Lim, Jong-Soo; Son, Woo Sung; Wu, Sangwook; Lee, Donghan. "Enhanced large-scale production and purification of recombinant human NUPR1 for detailed structural and functional insights." Protein Expr. Purif. 242, 106940-106940 (2026).
PubMed: 42092412

Assembly members:

Assembly members:
entity_1, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MATFPPATSAPQQPPGPEDE DSSLDESDLYSLAHSYLGGG GRKGRTKREAAANTNRPSPG GHERKLVTKLQNSERKKRGA RR

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	137
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Nurp1	1

Entities:

Entity 1, Nurp1 82 residues - Formula weight is not available

1

MET

ALA

THR

PHE

PRO

ALA

THR

SER

ALA

2

PRO

GLN

PRO

GLY

PRO

GLU

ASP

GLU

3

ASP

SER

LEU

ASP

GLU

SER

ASP

LEU

TYR

4

SER

LEU

ALA

HIS

SER

TYR

LEU

GLY

5

GLY

ARG

LYS

GLY

ARG

THR

LYS

ARG

GLU

ALA

6

ALA

ASN

THR

ASN

ARG

PRO

SER

PRO

GLY

7

GLY

HIS

GLU

ARG

LYS

LEU

VAL

THR

LYS

LEU

8

GLN

ASN

SER

GLU

ARG

LYS

ARG

GLY

ALA

9

ARG

Samples:

sample_1: HEPES 20 mM; sodium chloride 100 mM; sodium azide 0.02%; D2O 10%; Nupr1, [U-99% 13C; U-99% 15N], 6.2 mg/mL

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N TROSY	sample_1	isotropic	sample_conditions_1
3D HNCACB TROSY	sample_1	isotropic	sample_conditions_1

Software:

POKY - chemical shift assignment, data analysis, peak picking

NMRPipe - data analysis, processing

NMR spectrometers:

Bruker AVANCE III 700 MHz
Bruker AVANCE NEO 1200 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

BMRB Entry 53787