BMRB Entry 5376

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5376
MolProbity Validation Chart

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Title:
C-terminal peptide of alpha-subunit of transducin
Deposition date:
2002-05-15
Original release date:
2003-01-06
Authors:
Koenig, Bernd; Kontaxis, Georg; Mitchell, Drake; Louis, John; Litman, Burton; Bax, Ad
Citation:

Citation: Koenig, Bernd; Kontaxis, Georg; Mitchell, Drake; Louis, John; Litman, Burton; Bax, Ad. "Structure and Orientation of a G Protein Fragment in the Receptor Bound State from Residual Dipolar Couplings"  J. Mol. Biol. 322, 441-461 (2002).
PubMed: 12217702

Assembly members:

Assembly members:
Gt-alpha(340-350), polymer, 11 residues, Formula weight is not available
rhodopsin, polymer, 348 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: cow   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: GEV-S2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gt-alpha(340-350): IRENLKDSGLF
rhodopsin: MNGTEGPNFYVPFSNKTGVV RSPFEAPQYYLAEPWQFSML AAYMFLLIMLGFPINFLTLY VTVQHKKLRTPLNYILLNLA VADLFMVFGGFTTTLYTSLH GYFVFGPTGCNLEGFFATLG GEIALWSLVVLAIERYVVVC KPMSNFRFGENHAIMGVAFT WVMALACAAPPLVGWSRYIP EGMQCSCGIDYYTPHEETNN ESFVIYMFVVHFIIPLIVIF FCYGQLVFTVKEAAAQQQES ATTQKAEKEVTRMVIIMVIA FLICWLPYAGVAFYIFTHQG SDFGPIFMTIPAFFAKTSAV YNPVIYIMMNKQFRNCMVTT LCCGKNPLGDDEASTTVSKT ETSQVAPA

Data sets:
Data typeCount
1H chemical shifts81
13C chemical shifts36
15N chemical shifts10

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1S2 peptide1
2rhodopsin2

Entities:

Entity 1, S2 peptide 11 residues - Formula weight is not available

1   ILEARGGLUASNLEULYSASPSERGLYLEU
2   PHE

Entity 2, rhodopsin 348 residues - Formula weight is not available

1   METASNGLYTHRGLUGLYPROASNPHETYR
2   VALPROPHESERASNLYSTHRGLYVALVAL
3   ARGSERPROPHEGLUALAPROGLNTYRTYR
4   LEUALAGLUPROTRPGLNPHESERMETLEU
5   ALAALATYRMETPHELEULEUILEMETLEU
6   GLYPHEPROILEASNPHELEUTHRLEUTYR
7   VALTHRVALGLNHISLYSLYSLEUARGTHR
8   PROLEUASNTYRILELEULEUASNLEUALA
9   VALALAASPLEUPHEMETVALPHEGLYGLY
10   PHETHRTHRTHRLEUTYRTHRSERLEUHIS
11   GLYTYRPHEVALPHEGLYPROTHRGLYCYS
12   ASNLEUGLUGLYPHEPHEALATHRLEUGLY
13   GLYGLUILEALALEUTRPSERLEUVALVAL
14   LEUALAILEGLUARGTYRVALVALVALCYS
15   LYSPROMETSERASNPHEARGPHEGLYGLU
16   ASNHISALAILEMETGLYVALALAPHETHR
17   TRPVALMETALALEUALACYSALAALAPRO
18   PROLEUVALGLYTRPSERARGTYRILEPRO
19   GLUGLYMETGLNCYSSERCYSGLYILEASP
20   TYRTYRTHRPROHISGLUGLUTHRASNASN
21   GLUSERPHEVALILETYRMETPHEVALVAL
22   HISPHEILEILEPROLEUILEVALILEPHE
23   PHECYSTYRGLYGLNLEUVALPHETHRVAL
24   LYSGLUALAALAALAGLNGLNGLNGLUSER
25   ALATHRTHRGLNLYSALAGLULYSGLUVAL
26   THRARGMETVALILEILEMETVALILEALA
27   PHELEUILECYSTRPLEUPROTYRALAGLY
28   VALALAPHETYRILEPHETHRHISGLNGLY
29   SERASPPHEGLYPROILEPHEMETTHRILE
30   PROALAPHEPHEALALYSTHRSERALAVAL
31   TYRASNPROVALILETYRILEMETMETASN
32   LYSGLNPHEARGASNCYSMETVALTHRTHR
33   LEUCYSCYSGLYLYSASNPROLEUGLYASP
34   ASPGLUALASERTHRTHRVALSERLYSTHR
35   GLUTHRSERGLNVALALAPROALA

Samples:

sample_1: Gt-alpha(340-350), [U-15N; U-13C], 2.6 mM; rhodopsin 0.063 mM

sample_2: Gt-alpha(340-350) 2.6 mM

Ex-cond_1: pH: 6.6 na; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYnot availablenot availablenot available
2D 1H-1H TOCSY (15N separated)not availablenot availablenot available
2D 1H-15N HSQC (w/o 1H decoupling)not availablenot availablenot available
2D 1H-13C CT-HSQC (w/o 1H decoupling)not availablenot availablenot available

Software:

NMRPipe - raw spectral data processing, peak picking

NMR spectrometers:

  • Bruker DMX 600 MHz
  • Bruker DMX 750 MHz

Related Database Links:

PDB
DBJ BAB83621 BAB83621
GB AAA30674 AAA30675 ELR51227
PRF 0811197A 0901188A 0901212A 1001148A 0901212A 1001148A 0811197A 0901188A
REF NP_001014890 XP_004018583 XP_005902896 XP_006078962 XP_010860750 NP_001014890
SP P02699
TPG DAA16827
SWISS-PROT P02699
GenBank AAA30674 AAA30675
AlphaFold P02699

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks