BMRB Entry 53759

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53759

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Title:
SplB
Deposition date:
2026-05-05
Original release date:
2026-09-02
Authors:
Eisenmesser, Elan; Lee, Eunjeong
Citation:

Citation: Lee, Eunjeong; Redzic, Jasmina; Sarkar, Samrat; Rivkin, Daniel; Gordon, Blaine; Saviola, Anthony; Maroney, Sean; Thorstenson, John; Hansen, Kirk; Horswill, Alexander; Hamelberg, Donald; Eisenmesser, Elan. "N-terminal processing unlocks global dynamics for substrate engagement in Spl proteases "  Sci. Adv. 12, eaeh5197-eaeh5197 (2026).
PubMed: 42497254

Assembly members:

Assembly members:
entity_1, polymer, 204 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Staphylococcus aureus   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: ENNVTKVKDTNIFPYTGVVA FKSATGFVVGKNTILTNKHV SKNYKVGDRITAHPNSDKGN GGIYSIKKIINYPGKEDVSV IQVEERAIERGPKGFNFNDN VTPFKYAAGAKAGERIKVIG YPHPYKNKYVLYESTGPVMS VEGSSIVYSAHTESGNSGSP VLNSNNELVGIHFASDVKND DNRNAYGVYFTPEIKKFIAE NIDK

Data sets:
Data typeCount
13C chemical shifts360
15N chemical shifts180
1H chemical shifts180

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1SplB1

Entities:

Entity 1, SplB 204 residues - Formula weight is not available

1   GLUASNASNVALTHRLYSVALLYSASPTHR
2   ASNILEPHEPROTYRTHRGLYVALVALALA
3   PHELYSSERALATHRGLYPHEVALVALGLY
4   LYSASNTHRILELEUTHRASNLYSHISVAL
5   SERLYSASNTYRLYSVALGLYASPARGILE
6   THRALAHISPROASNSERASPLYSGLYASN
7   GLYGLYILETYRSERILELYSLYSILEILE
8   ASNTYRPROGLYLYSGLUASPVALSERVAL
9   ILEGLNVALGLUGLUARGALAILEGLUARG
10   GLYPROLYSGLYPHEASNPHEASNASPASN
11   VALTHRPROPHELYSTYRALAALAGLYALA
12   LYSALAGLYGLUARGILELYSVALILEGLY
13   TYRPROHISPROTYRLYSASNLYSTYRVAL
14   LEUTYRGLUSERTHRGLYPROVALMETSER
15   VALGLUGLYSERSERILEVALTYRSERALA
16   HISTHRGLUSERGLYASNSERGLYSERPRO
17   VALLEUASNSERASNASNGLULEUVALGLY
18   ILEHISPHEALASERASPVALLYSASNASP
19   ASPASNARGASNALATYRGLYVALTYRPHE
20   THRPROGLUILELYSLYSPHEILEALAGLU
21   ASNILEASPLYS

Samples:

sample_1: 1, [U-100% 13C; U-100% 15N], 500 ± 50 uM

sample_conditions_1: ionic strength: 0.05 M; pH: 6; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D CBCACONHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

CcpNMR v2 - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks