BMRB Entry 53723

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53723

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry

Title:
Solution assignment of CD7opt bound to K12 (SECTM1)
Deposition date:
2026-04-15
Original release date:
2026-04-30
Authors:
Miles, Uri; Wu, Hongwei; Mcshan, Andrew
Citation:

Citation: Miles, Uri; Wu, Hongwei; Mcshan, Andrew. "A structural basis for T cell co-stimulation by the CD7 immunoreceptor and stimulatory ligand K12"  .

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, Formula weight is not available
entity_2, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-22(b)+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AQEVQQSPHCTTVPVGASVN ITCSTSGGLRGIYLRQLGPQ PQDIIYYEDGVVPTTDRRFR GRIDFSGSQDNLTITMHRLQ LSDTGTYTCQAITEVNVYGS GTLVLVTEEQSQGWHRCSDA
entity_2: QNEGWDSPICTEGVVSVSWG ENTVMSCNISNAFSHVNIKL RAHGQESAIFNEVAPGYFSR DGWQLQVQGGVAQLVIKGAR DSHAGLYMWHLVGHQRNNRQ VTLEVSGAE

Data sets:
Data typeCount
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CD7opt1
2K122

Entities:

Entity 1, CD7opt 120 residues - Formula weight is not available

1   ALAGLNGLUVALGLNGLNSERPROHISCYS
2   THRTHRVALPROVALGLYALASERVALASN
3   ILETHRCYSSERTHRSERGLYGLYLEUARG
4   GLYILETYRLEUARGGLNLEUGLYPROGLN
5   PROGLNASPILEILETYRTYRGLUASPGLY
6   VALVALPROTHRTHRASPARGARGPHEARG
7   GLYARGILEASPPHESERGLYSERGLNASP
8   ASNLEUTHRILETHRMETHISARGLEUGLN
9   LEUSERASPTHRGLYTHRTYRTHRCYSGLN
10   ALAILETHRGLUVALASNVALTYRGLYSER
11   GLYTHRLEUVALLEUVALTHRGLUGLUGLN
12   SERGLNGLYTRPHISARGCYSSERASPALA

Entity 2, K12 109 residues - Formula weight is not available

1   GLNASNGLUGLYTRPASPSERPROILECYS
2   THRGLUGLYVALVALSERVALSERTRPGLY
3   GLUASNTHRVALMETSERCYSASNILESER
4   ASNALAPHESERHISVALASNILELYSLEU
5   ARGALAHISGLYGLNGLUSERALAILEPHE
6   ASNGLUVALALAPROGLYTYRPHESERARG
7   ASPGLYTRPGLNLEUGLNVALGLNGLYGLY
8   VALALAGLNLEUVALILELYSGLYALAARG
9   ASPSERHISALAGLYLEUTYRMETTRPHIS
10   LEUVALGLYHISGLNARGASNASNARGGLN
11   VALTHRLEUGLUVALSERGLYALAGLU

Samples:

sample_1: protein, [U-13C; U-15N; U-2H], 0.13 mM; K12 0.13 mM; D2O, [U-100% 2H], 10%; sodium azide 0.02%; sodium phosphate 20 mM; EDTA 1 mM; sodium chloride 100 mM

sample_conditions_1: ionic strength: 120 mM; pH: 6.5; pressure: 1 atm; temperature: 298.15 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe - processing

NMRFAM-SPARKY - data analysis

NMR spectrometers:

  • Bruker AVANCE III 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks