BMRB Entry 53717
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53717
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Tran, Thi Thanh Quynh; Larda, Sacha; Nieves, Cecilia; Veyrier, Frederic; Calmettes, Charles; Doucet, Nicolas. "Engineering human RNase 7 with an eosinophil RNase segment reveals determinants of cytotoxic and antimicrobial activity
" J. Biol. Chem. 302, 113189-113189 (2026).
PubMed: 42203172
Assembly members:
entity_1, polymer, 128 residues, 14633.02 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pJExpress414
Entity Sequences (FASTA):
entity_1: KPKGMTSSQWFKIQHMQPSP
QACNSAMKNINKHTKRCKDL
NTFLHEPFSSVAATCQTPKI
ACKNGDKNCHQSHGAVSLTM
CKLTSGKHPNCRYKEKRQNK
SYVVACKPPQKKDSQQFHLV
PVHLDRVL
- assigned_chemical_shifts
- spectral_peak_list
| Data type | Count |
| 13C chemical shifts | 233 |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | RNase 7 monomer | 1 |
| 2 | zinc ion, 1 | 2 |
| 3 | zinc ion, 2 | 2 |
| 4 | zinc ion, 3 | 2 |
| 5 | zinc ion, 4 | 2 |
Entities:
Entity 1, RNase 7 monomer 128 residues - 14633.02 Da.
| 1 | LYS | PRO | LYS | GLY | MET | THR | SER | SER | GLN | TRP | ||||
| 2 | PHE | LYS | ILE | GLN | HIS | MET | GLN | PRO | SER | PRO | ||||
| 3 | GLN | ALA | CYS | ASN | SER | ALA | MET | LYS | ASN | ILE | ||||
| 4 | ASN | LYS | HIS | THR | LYS | ARG | CYS | LYS | ASP | LEU | ||||
| 5 | ASN | THR | PHE | LEU | HIS | GLU | PRO | PHE | SER | SER | ||||
| 6 | VAL | ALA | ALA | THR | CYS | GLN | THR | PRO | LYS | ILE | ||||
| 7 | ALA | CYS | LYS | ASN | GLY | ASP | LYS | ASN | CYS | HIS | ||||
| 8 | GLN | SER | HIS | GLY | ALA | VAL | SER | LEU | THR | MET | ||||
| 9 | CYS | LYS | LEU | THR | SER | GLY | LYS | HIS | PRO | ASN | ||||
| 10 | CYS | ARG | TYR | LYS | GLU | LYS | ARG | GLN | ASN | LYS | ||||
| 11 | SER | TYR | VAL | VAL | ALA | CYS | LYS | PRO | PRO | GLN | ||||
| 12 | LYS | LYS | ASP | SER | GLN | GLN | PHE | HIS | LEU | VAL | ||||
| 13 | PRO | VAL | HIS | LEU | ASP | ARG | VAL | LEU |
Entity 2, zinc ion, 1 - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: Human ribonuclease 7, [U-99% 13C; U-99% 15N], 269 uM; sodium acetate 15 mM
sample_conditions_1: ionic strength: 0.015 M; pH: 5.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRbox - chemical shift assignment, peak picking, processing
NMR spectrometers:
- Bruker AVANCE III 800 MHz
Related Database Links:
| PDB | |
| NCBI | 84659 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks