BMRB Entry 53715
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53715
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Dengler, Alisa; Alderson, Reid. "Bromodomain dimers: a case study of BRD4 and family-wide AlphaFold predictions" Struct. Dyn. ., .-. (2026).
Assembly members:
entity_1, polymer, 116 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: HS_Brd4_BD2_pET-29b(+)
Entity Sequences (FASTA):
entity_1: EKSSKVSEQLKCCSGILKEM
FAKKHAAYAWPFYKPVDVEA
LGLHDYCDIIKHPMDMSTIK
SKLEAREYRDAQEFGADVRL
MFSNCYKYNPPDHEVVAMAR
KLQDVFEMRFAKMPDE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 313 |
| 15N chemical shifts | 101 |
| 1H chemical shifts | 101 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | BRD4 BD2 | 1 |
Entities:
Entity 1, BRD4 BD2 116 residues - Formula weight is not available
345-460
| 1 | GLU | LYS | SER | SER | LYS | VAL | SER | GLU | GLN | LEU | ||||
| 2 | LYS | CYS | CYS | SER | GLY | ILE | LEU | LYS | GLU | MET | ||||
| 3 | PHE | ALA | LYS | LYS | HIS | ALA | ALA | TYR | ALA | TRP | ||||
| 4 | PRO | PHE | TYR | LYS | PRO | VAL | ASP | VAL | GLU | ALA | ||||
| 5 | LEU | GLY | LEU | HIS | ASP | TYR | CYS | ASP | ILE | ILE | ||||
| 6 | LYS | HIS | PRO | MET | ASP | MET | SER | THR | ILE | LYS | ||||
| 7 | SER | LYS | LEU | GLU | ALA | ARG | GLU | TYR | ARG | ASP | ||||
| 8 | ALA | GLN | GLU | PHE | GLY | ALA | ASP | VAL | ARG | LEU | ||||
| 9 | MET | PHE | SER | ASN | CYS | TYR | LYS | TYR | ASN | PRO | ||||
| 10 | PRO | ASP | HIS | GLU | VAL | VAL | ALA | MET | ALA | ARG | ||||
| 11 | LYS | LEU | GLN | ASP | VAL | PHE | GLU | MET | ARG | PHE | ||||
| 12 | ALA | LYS | MET | PRO | ASP | GLU |
Samples:
sample_1: BRD4 BD2, [U-13C; U-15N], 0.2 mM; MES 25 mM; NaCl 50 mM; TCEP 1 mM; EDTA 0.5 mM
sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CA)CO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
TOPSPIN - collection
NMRPipe - processing
NMRFAM-SPARKY - chemical shift assignment
NMR spectrometers:
- Bruker AVANCE III 500 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks