Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53710

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: R1, R2, HetNOE of HBx(1-120) in 190 mM Urea
Published: 2026-05-26

Title:

R1, R2, HetNOE of HBx(1-120) in 190 mM Urea

Deposition date:

2026-04-07

Original release date:

2026-05-26

Authors:

Clavier, Alexis; Shida, Toshinobu; Dromer, Maxim; Holzinger, Julian; Schutz, Anne

Citation:

Citation: Clavier, Alexis; Shida, Toshinobu; Dromer, Maxim; Holzinger, Julian; Schutz, Anne. "HBV HBx protein masks epigenetic reader Spindlin1 via an inter-molecular zinc finger to subvert transcriptional control" Nat. Commun. ., .-. (2026).
PubMed: 42185260

Assembly members:

Assembly members:
entity_1, polymer, 120 residues, Formula weight is not available

Natural source:

Natural source: Common Name: hepatitis B Taxonomy ID: 10407 Superkingdom: Viruses Kingdom: not available Genus/species: Orthohepadnavirus hepatitis B

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet28a(+)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: MAARLCCQLDPARDVLCLRP VGAESCGRPFSGSLGTLSSP SPSAVPTDHGAHLSLRGLPV CAFSSAGPCALRFTSARRME TTVNAHQILPKVLHKRTLGL SAMSTTDLEAYFKDCLFKDW

Data sets:

heteronucl_NOEs
- heteronucl_NOEs_1
- heteronucl_NOEs_2
heteronucl_T1_relaxation
- heteronucl_T1_relaxation_1
- heteronucl_T1_relaxation_2
heteronucl_T2_relaxation
- heteronucl_T2_relaxation_1
- heteronucl_T2_relaxation_2

Data type	Count
T1 relaxation values	207
T2 relaxation values	206
heteronuclear NOE values	207

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HBx 1-120	1

Entities:

Entity 1, HBx 1-120 120 residues - Formula weight is not available

short isoform HBx genotype D

1	MET	ALA	ALA	ARG	LEU	CYS	CYS	GLN	LEU	ASP
2	PRO	ALA	ARG	ASP	VAL	LEU	CYS	LEU	ARG	PRO
3	VAL	GLY	ALA	GLU	SER	CYS	GLY	ARG	PRO	PHE
4	SER	GLY	SER	LEU	GLY	THR	LEU	SER	SER	PRO
5	SER	PRO	SER	ALA	VAL	PRO	THR	ASP	HIS	GLY
6	ALA	HIS	LEU	SER	LEU	ARG	GLY	LEU	PRO	VAL
7	CYS	ALA	PHE	SER	SER	ALA	GLY	PRO	CYS	ALA
8	LEU	ARG	PHE	THR	SER	ALA	ARG	ARG	MET	GLU
9	THR	THR	VAL	ASN	ALA	HIS	GLN	ILE	LEU	PRO
10	LYS	VAL	LEU	HIS	LYS	ARG	THR	LEU	GLY	LEU
11	SER	ALA	MET	SER	THR	THR	ASP	LEU	GLU	ALA
12	TYR	PHE	LYS	ASP	CYS	LEU	PHE	LYS	ASP	TRP

Samples:

sample_1: HBx isoform 1-120, [U-98% 15N], 50 uM; urea 190 mM; HEPES 50 mM; sodium chloride 150 mM; L-arginine 125 mM

sample_conditions_1: pH: 7.4; temperature: 277 K

Experiments:

Name	Sample	Sample state	Sample conditions
T1/R1 relaxation	sample_1	isotropic	sample_conditions_1
T2/R2 relaxation	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1
T1/R1 relaxation	sample_1	isotropic	sample_conditions_1
T2/R2 relaxation	sample_1	isotropic	sample_conditions_1
1H-15N heteronoe	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR vCcpNmr AnalysisAssign (3.3.2.3) - data analysis

NMRPipe v11.7 - processing

NMR spectrometers:

Bruker AVANCE III 800 MHz
Bruker AVANCE NEO 1200 MHz

1	MET	ALA	ALA	ARG	LEU	CYS	CYS	GLN	LEU	ASP
2	PRO	ALA	ARG	ASP	VAL	LEU	CYS	LEU	ARG	PRO
3	VAL	GLY	ALA	GLU	SER	CYS	GLY	ARG	PRO	PHE
4	SER	GLY	SER	LEU	GLY	THR	LEU	SER	SER	PRO
5	SER	PRO	SER	ALA	VAL	PRO	THR	ASP	HIS	GLY
6	ALA	HIS	LEU	SER	LEU	ARG	GLY	LEU	PRO	VAL
7	CYS	ALA	PHE	SER	SER	ALA	GLY	PRO	CYS	ALA
8	LEU	ARG	PHE	THR	SER	ALA	ARG	ARG	MET	GLU
9	THR	THR	VAL	ASN	ALA	HIS	GLN	ILE	LEU	PRO
10	LYS	VAL	LEU	HIS	LYS	ARG	THR	LEU	GLY	LEU
11	SER	ALA	MET	SER	THR	THR	ASP	LEU	GLU	ALA
12	TYR	PHE	LYS	ASP	CYS	LEU	PHE	LYS	ASP	TRP

1	MET	ALA	ALA	ARG	LEU	CYS	CYS	GLN	LEU	ASP
2	PRO	ALA	ARG	ASP	VAL	LEU	CYS	LEU	ARG	PRO
3	VAL	GLY	ALA	GLU	SER	CYS	GLY	ARG	PRO	PHE
4	SER	GLY	SER	LEU	GLY	THR	LEU	SER	SER	PRO
5	SER	PRO	SER	ALA	VAL	PRO	THR	ASP	HIS	GLY
6	ALA	HIS	LEU	SER	LEU	ARG	GLY	LEU	PRO	VAL
7	CYS	ALA	PHE	SER	SER	ALA	GLY	PRO	CYS	ALA
8	LEU	ARG	PHE	THR	SER	ALA	ARG	ARG	MET	GLU
9	THR	THR	VAL	ASN	ALA	HIS	GLN	ILE	LEU	PRO
10	LYS	VAL	LEU	HIS	LYS	ARG	THR	LEU	GLY	LEU
11	SER	ALA	MET	SER	THR	THR	ASP	LEU	GLU	ALA
12	TYR	PHE	LYS	ASP	CYS	LEU	PHE	LYS	ASP	TRP

BMRB Entry 53710