Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53698

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Name: Backbone 1H, 13C, and 15N chemical shift assignments for the chromoshadow domain and C-terminal extension (residue 112-191) of human heterochromatin protein 1alpha (HP1alpha).
Published: 2026-06-02

Title:

Backbone 1H, 13C, and 15N chemical shift assignments for the chromoshadow domain and C-terminal extension (residue 112-191) of human heterochromatin protein 1alpha (HP1alpha).

Deposition date:

2026-03-27

Original release date:

2026-06-02

Authors:

Bhakta, Rushabh; Debelouchina, Galia

Citation:

Citation: Abasi, Lannah; Tea, Challana; Bhakta, Rushabh; Le, Donald; Phan, Tien; Mittal, Jeetain; Ferguson, Cole; Debelouchina, Galia. "Ki-67 regulates heterochromatin organization in neurons and forms condensates with heterochromatic components" Nat. Commun. ., .-..

Assembly members:

Assembly members:
entity_1, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET (2B-T)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: DIARGFERGLEPEKIIGATD SCGDLMFLMKWKDTDEADLV LAKEANVKCPQIVIAFYEER LTWHAYPEDAENKEKETAKS

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	98
15N chemical shifts	73
1H chemical shifts	73

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HP1alpha CSD-CTE	1

Entities:

Entity 1, HP1alpha CSD-CTE 80 residues - Formula weight is not available

1	ASP	ILE	ALA	ARG	GLY	PHE	GLU	ARG	GLY	LEU
2	GLU	PRO	GLU	LYS	ILE	ILE	GLY	ALA	THR	ASP
3	SER	CYS	GLY	ASP	LEU	MET	PHE	LEU	MET	LYS
4	TRP	LYS	ASP	THR	ASP	GLU	ALA	ASP	LEU	VAL
5	LEU	ALA	LYS	GLU	ALA	ASN	VAL	LYS	CYS	PRO
6	GLN	ILE	VAL	ILE	ALA	PHE	TYR	GLU	GLU	ARG
7	LEU	THR	TRP	HIS	ALA	TYR	PRO	GLU	ASP	ALA
8	GLU	ASN	LYS	GLU	LYS	GLU	THR	ALA	LYS	SER

Samples:

sample_1: HP1alpha CSD-CTE, [U-99% 13C; U-99% 15N], 640 uM; potassium chloride 75 mM; HEPES 20 mM; TCEP 1 mM; sodium azide 0.05%

sample_conditions_1: pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1

Software:

POKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 800 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks

1	ASP	ILE	ALA	ARG	GLY	PHE	GLU	ARG	GLY	LEU
2	GLU	PRO	GLU	LYS	ILE	ILE	GLY	ALA	THR	ASP
3	SER	CYS	GLY	ASP	LEU	MET	PHE	LEU	MET	LYS
4	TRP	LYS	ASP	THR	ASP	GLU	ALA	ASP	LEU	VAL
5	LEU	ALA	LYS	GLU	ALA	ASN	VAL	LYS	CYS	PRO
6	GLN	ILE	VAL	ILE	ALA	PHE	TYR	GLU	GLU	ARG
7	LEU	THR	TRP	HIS	ALA	TYR	PRO	GLU	ASP	ALA
8	GLU	ASN	LYS	GLU	LYS	GLU	THR	ALA	LYS	SER

1	ASP	ILE	ALA	ARG	GLY	PHE	GLU	ARG	GLY	LEU
2	GLU	PRO	GLU	LYS	ILE	ILE	GLY	ALA	THR	ASP
3	SER	CYS	GLY	ASP	LEU	MET	PHE	LEU	MET	LYS
4	TRP	LYS	ASP	THR	ASP	GLU	ALA	ASP	LEU	VAL
5	LEU	ALA	LYS	GLU	ALA	ASN	VAL	LYS	CYS	PRO
6	GLN	ILE	VAL	ILE	ALA	PHE	TYR	GLU	GLU	ARG
7	LEU	THR	TRP	HIS	ALA	TYR	PRO	GLU	ASP	ALA
8	GLU	ASN	LYS	GLU	LYS	GLU	THR	ALA	LYS	SER

BMRB Entry 53698