BMRB Entry 53692
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR53692
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Wos, Amadeusz; Gudanis-Sobocinska, Dorota; Zielinska, Karolina; Pawlowicz-Perczak, Aleksandra; Pasternak, Karol; Gdaniec, Zofia; Andralojc, Witold. "How loop-lengths shape topological preferences of two-tetrad G-quadruplexes" .
Assembly members:
entity_1, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: GGTTTTGGTTTTGGTTGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 8 |
| 1H chemical shifts | 175 |
| 31P chemical shifts | 17 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | G-quadruplex 5'-GGTTGGTTTGGTTGG-3' | 1 |
Entities:
Entity 1, G-quadruplex 5'-GGTTGGTTTGGTTGG-3' 18 residues - Formula weight is not available
| 1 | DG | DG | DT | DT | DT | DT | DG | DG | DT | DT | ||||
| 2 | DT | DT | DG | DG | DT | DT | DG | DG |
Samples:
sample_1: G-quadruplex 5'-GGTTTTGGTTTTGGTTGG-3' 2-3 mM; potassium chloride 150 mM; potassium phosphate 10 mM
sample_conditions_1: ionic strength: 0.16 M; pH: 7.6; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-13C HMBC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-31P COSY | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRFAM-SPARKY - chemical shift assignment
TOPSPIN - collection
NMR spectrometers:
- Bruker AVANCE III 700 MHz