BMRB Entry 5368

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5368

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for hARF1
Deposition date:
2002-05-09
Original release date:
2005-11-14
Authors:
Amor, Juan Carlos; Seidel, Ronald; Tian, Fang; Kahn, Richard; Prestegard, James
Citation:

Citation: Amor, Juan Carlos; Seidel, Ronald; Tian, Fang; Kahn, Richard; Prestegard, James. "Letter to the Editor: 1H, 15N, and 13C Assignments of Full Length Human ADP Ribosylation Factor 1 (ARF1) using Triple Resonance Connectivities and Dipolar Couplings"  J. Biomol. NMR 23, 253-254 (2002).
PubMed: 12238602

Assembly members:

Assembly members:
ADP Ribosylation Factor 1, polymer, 180 residues, 20562 Da.
GUANOSINE-5'-DIPHOSPHATE, non-polymer, 443.201 Da.
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ADP Ribosylation Factor 1: GNIFANLFKGLFGKKEMRIL MVGLDAAGKTTILYKLKLGE IVTTIPTIGFNVETVEYKNI SFTVWDVGGQDKIRPLWRHY FQNTQGLIFVVDSNDRERVN EAREELMRMLAEDELRDAVL LVFANKQDLPNAMNAAEITD KLGLHSLRHRNWYIQATCAT SGDGLYEGLDWLSNQLRNQK

Data sets:
Data typeCount
1H chemical shifts164
13C chemical shifts474
15N chemical shifts164

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hARF1 monomer1
2GUANOSINE-5'-DIPHOSPHATE2
3MG 2+3

Entities:

Entity 1, hARF1 monomer 180 residues - 20562 Da.

1   GLYASNILEPHEALAASNLEUPHELYSGLY
2   LEUPHEGLYLYSLYSGLUMETARGILELEU
3   METVALGLYLEUASPALAALAGLYLYSTHR
4   THRILELEUTYRLYSLEULYSLEUGLYGLU
5   ILEVALTHRTHRILEPROTHRILEGLYPHE
6   ASNVALGLUTHRVALGLUTYRLYSASNILE
7   SERPHETHRVALTRPASPVALGLYGLYGLN
8   ASPLYSILEARGPROLEUTRPARGHISTYR
9   PHEGLNASNTHRGLNGLYLEUILEPHEVAL
10   VALASPSERASNASPARGGLUARGVALASN
11   GLUALAARGGLUGLULEUMETARGMETLEU
12   ALAGLUASPGLULEUARGASPALAVALLEU
13   LEUVALPHEALAASNLYSGLNASPLEUPRO
14   ASNALAMETASNALAALAGLUILETHRASP
15   LYSLEUGLYLEUHISSERLEUARGHISARG
16   ASNTRPTYRILEGLNALATHRCYSALATHR
17   SERGLYASPGLYLEUTYRGLUGLYLEUASP
18   TRPLEUSERASNGLNLEUARGASNGLNLYS

Entity 2, GUANOSINE-5'-DIPHOSPHATE 1 residues - C10 H15 N5 O11 P2 - 443.201 Da.

1   GDP

Entity 3, MG 2+ - Mg - 24.305 Da.

1   MG

Samples:

sample_1: ADP Ribosylation Factor 1 0.5 mM; GUANOSINE-5'-DIPHOSPHATE mM; magnesium mM

Ex-cond_1: pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCOnot availablenot availablenot available
CBCACONHnot availablenot availablenot available
HNCACBnot availablenot availablenot available

Software:

FELIX v2000 -

NMR spectrometers:

  • Varian INOVA 600 MHz

Related Database Links:

PDB
DBJ BAA13490 BAE01583 BAE28566 BAE28685 BAE29300
EMBL CAG03028 CAG31143 CAJ81862 CAL38139 CAL38339
GB AAA30361 AAA35511 AAA35512 AAA35552 AAA40685
PRF 2004472A
REF NP_001001905 NP_001006352 NP_001019397 NP_001019398 NP_001019399
SP P51643 P84077 P84078 P84079 P84080
TPG DAA28323 DAA29912 DAA31858
AlphaFold P51643 P84077 P84078 P84079 P84080

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks